茶叶及茶多糖中氟测定前处理方法的比较研究.pdf

茶叶科学2015，35（2）：145~150
JournalofTeaScience投稿平台：http://cykk.cbpt.cnki.net

茶叶及茶多糖中氟测定前处理方法的比较研究

*
朱晓静，房峰祥，张月华，倪德江，陈玉琼

园艺植物生物学教育部重点实验室，华中农业大学园艺林学学院，湖北武汉430070

摘要：以不同品种茶树鲜叶及茶多糖为材料，比较了酸浸提、沸水浸提和碱熔灰化前处理对氟离子选择电极
法测定氟含量的影响。结果表明，无论对茶叶还是茶多糖，碱熔灰化处理所测氟含量均极显著高于酸浸提和
沸水浸提法（P<0.01）。茶叶水浸提法氟含量显著或极显著高于酸浸提法（P<0.05，P<0.01）。对碱熔灰化-
氟离子电极法进行精密度及回收率实验，结果表明茶叶和多糖中氟的回收率分别达到91.07%~94.40%和
83.04%~90.32%，而RSD分别为1.44%~2.54%和0.68%~1.03%，说明该方法稳定性好，精密度高，检测结果可
靠，更能真实反映茶叶及茶多糖的氟含量，适宜于茶叶及茶提取物全氟的测定。
关键词：茶叶；茶多糖；氟含量；预处理方法；氟离子选择电极法
中图分类号：S571.1；O652文献标识码：A文章编号：1000-369X（2015）02-145-06

DOI:10.13305/j.cnki.jts.2015.02.006

ComparisononthePretreatmentMethodsofTeaandTea
PolysaccharidesforDeterminationofFluorineContent

ZHUXiaojing,FANGFengxiang,ZHANGYuehua,NIDejiang,CHENYuqiong*

KeyLaboratoryofHorticulturalPlantBiology,MinistryofEducation/CollegeofHorticultureandForestrySciences,Huazhong
AgriculturalUniversity,Wuhan430070,China

Abstract:Theinfluenceofsamplepretreatmentmethodsincludingboilingwaterextraction(BWE)，acidicsolution
extraction(ASE)andalkalizingandburningtoash(ABA)onfluorinecontentinteaandteapolysaccharideswas
investigated.TheresultsshowedthatthefluorinecontentsofteaandteapolysaccharidepretreatedwithABAwere
muchhigherthanthatofBWEandASEmethod(P<0.01).ThefluorinecontentofteapretreatedwithBWEwas
significantlyhigherthanASE(P<0.05,P<0.01).ThefurthertestonprecisionandrecoveryofABAandfluorideion
selectiveelectrodemethodwasinvestigated.Resultsshowedthattherecoveryoffluorineinteaandtea
polysaccharidewere91.07%-94.40%and83.04%-90.32%,respectively,withRSDof1.44%-2.54%forteaand
0.68%-1.03%forteapolysaccharide,whichindicatedtheresultoffluorinedeterminationbythismethodcould
representtherealfluorinecontentinteaandteapolysaccharidewithaccuracy,reliabilityandstability.Itwasalso
suggestedthattheABAandfluorideionselectiveelectrodemethodcouldbemoresuitablefordeterminationoftotal
fluorineinteaandteaextracts.
Keywords:tea,teapolysaccharide,fluorinecontent,pretreatment,fluorideionselectiveelectrode

氟对人体具有双重作用，适量摄入