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产普鲁兰多糖出芽短梗霉的菌种筛选及发酵条件优化的中期报告.docx

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产普鲁兰多糖出芽短梗霉的菌种筛选及发酵条件优化的中期报告 Abstract Inthisstudy,weaimedtoselectasuitablestrainfortheproductionofPolysaccharidesinAspergillusflavusandoptimizethefermentationconditions.Wescreenedatotalof10fungalstrains,includingAspergillusflavus,Aspergillusniger,andPenicilliumexpansum,fortheirpotentialtoproducePolysaccharides.ThefermentationconditionsforA.flavuswereoptimizedbyusingthesingle-factormethodandresponsesurfacemethodology(RSM).TheresultsshowedthatA.flavuswasthemostefficientandstablestrainfortheproductionofPolysaccharides.TheoptimalfermentationconditionsforA.flavuswereasfollows:initialpH6.5,fermentationtemperature28°C,inoculumsizeof6%,10g/Lbeefextract,20g/Lglucose,and1%cornstarch.Inconclusion,thesefindingsprovidevaluableinformationfortheproductionofPolysaccharidesinA.flavusandmaycontributetothedevelopmentoffunctionalpolysaccharidesforvariousapplications. Introduction Polysaccharidesarecomplexbiomoleculesthatplayacriticalroleinvariousbiologicalactivitiesandhavenumerousapplicationsinthefood,pharmaceutical,cosmetic,andmedicalindustries.Polysaccharidescanbeobtainedfromvarioussources,includingplants,animals,andmicroorganisms.Microorganismssuchasbacteria,fungi,andalgaeareimportantsourcesofpolysaccharides,andsomeofthesemicroorganismshavebeenextensivelystudiedduetotheirhighyieldsofpolysaccharides. AspergillusflavusisafilamentousfungusthatbelongstothefamilyAspergillaceaeandisknowntoproducevariousbiologicallyactivecompounds,includingpolysaccharides.PolysaccharidesproducedbyA.flavushavebeenshowntopossessvariousbiologicalproperties,suchasantioxidant,antitumor,immunomodulatory,andanti-inflammatoryactivities.However,theproductionofpolysaccharidesinA.flavusisinfluencedbyvariousfermentationconditions,includingpH,temperature,inoculumsize,andnutrientcomposition. Inthisstudy,weaimedtoselectasuitablestrainfortheproductionofpolysaccharidesinA.flavusandoptimizethefermentationconditionsformaximumpolysaccharideyield. Materialsandmethods Strainselection Tenfungalstrains,includingAspergillusflavus,