生物化学实验指导.doc

2008年3月

湖北师范学院生命科学学院生物化学实验指导

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目录

前言···························································································································2

生物化学实验守则········································································································3

实验一实验基本知识及溶液的配制··········································································5

实验二蛋白质及氨基酸的呈色反应··········································································9

实验三蛋白质的等电点测定和沉淀反应·································································13

实验四蛋白质的定量测定方法················································································17

实验五离子交换柱层析法分离氨基酸·····································································25

实验六凝胶层析法和SDS聚丙烯酰胺凝胶电泳法—蛋白质的分子量测定··········27

实验七酶的特性········································································································34

实验八底物浓度对酶促反应速度的影响·································································38

实验九酪氨酸酶的提取及其催化活性研究·····························································40

实验十核糖核酸的提取与组分鉴定及核酸含量的测定·········································44

实验十一果蔬维生素C含量测定及其分析······························································47

实验十二小麦萌发前后淀粉酶活力的比较·····························································50

实验十三脂肪酸的β－氧化······················································································53

实验十四氨基移换反应（一）——血液中转氨酶活力的测定·····························55

实验十五氨基移换反应（二）――谷丙转氨酶活性的鉴定·································57

实验十六发酵过程中无机磷的利用········································································59

参考文献······················································································································62

湖北师范学院生命科学学院生物化学实验指导

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前言

近几年来，随着生物化学学科的迅猛发展，教学内容更新较快。生化实验

被作为主干基础课列入了高等院校生命科学各专业的教学计划中，并且实验课

单独作为一门课程开出。为了提高教学水平，反映出本学科更具科学性、先进

性和实用性的实验内容，根据教学大纲的要求和课程设置，同时根据本校教学

实验条件，在参阅中外相关文献，学习兄弟院校的教学经验和资料的基础上，

编写了本实验讲义。

本书为本校生