天芪降糖胶囊降糖调脂的机制[1].doc

应用基因芯片探讨天芪降糖胶囊降糖调脂的机制
张茜肖新华王彤李文慧袁涛孙晓方孙琦向红丁王姮
【摘要】目的探讨天芪降糖胶囊改善血糖和血脂的机制。方法选用KKAy小鼠16只，随机分为天芪组（给予1.5g/kg·d的天芪降糖胶囊粉末悬浊液）和对照组（给予等体积生理盐水），灌胃4周。测定小鼠FBG、血脂和FINS，并行OGTT实验。取小鼠骨骼肌进行RT-PCR基因芯片实验。结果天芪组FBG、OGTT曲线下面积（AUC）、HOMA-IR、TC和TG均比对照组显著降低（P<0.05或P<0.01)。基因芯片显示天芪组GLUT4、MAPK8、MAPK14和PPARα上调,PPARγ、PGC、CEBP、TNFα、NFκB、ICAM-1和IL-6下调。结论天芪降糖胶囊能有效降低KKAy小鼠FBG，调节脂代谢。其血糖降低机制可能与MAPK通路和GLUT4上调有关；另外可能通过下调炎症因子水平改善胰岛素抵抗。血脂改善可能与PPARα上调有关。
【关键词】天芪降糖胶囊；KKAy小鼠；基因芯片；糖尿病；脂代谢紊乱
ThemechanismofTianqicapsuleonglucoseandlipidregulatingusingRTPCRarrayZHANGQian,XIAOXin-Hua,WANGTong,etal.DepartmentofEndocrinology,PekingUnionMedicalCollegeHospital,PekingUnionMedicalCollege,Beijing100730,China
【Abstract】ObjectiveToexploretheeffectsofTianqicapsuleontheFBG,lipidmetabolismandFINSinKKAymiceandtoinvestigatetheirpossibletherapeuticmechanisms.MethodsKKAymicewererandomlydividedintotwogroups:Tianqigroup(treatedwith1.5g/kg·dTianqi)andcontrolgroup(treatedwiththesamevolumeofNS).Treatmentlastedfor4weeks.FBG,TC,TG,HDL-c,LDL-candFINSweredetected.TheOGTTtestwasdone.RTPCRarrayexperimentwasdonewithskeletalmuscle.ResultsTianqicapsulecoulddecreaseFBG,glucoseareaunderthecurve(AUC),HOMA-IR,TCandTG,comparedwiththoseofcontrolgroup（P<0.05orP<0.01).RTPCRarrayindicatedthatTianqiup-regulatedtheexpressionofGLUT4、MAPK8、MAPK14andPPARα，whiletheexpressionofPPARγ、PGC、CEBP、TNFα、NFκB、ICAM-1andIL-6weredown-regulated.ConclusionsTianqicapsulepossessesthefunctionsofregulatingthelevelsofbloodglucoseandlipidsinKKAymice.Andtheglucose-lowingmechanismsarecorrelatedwithGLUT4andMAPKpathway,andPPARαup-regulatedinvolveswithlipid-regulating.Moreover,TianqicapsulecandecreaseHYPERLINK"http://dict.cnki.net/dict_result.aspx?searchword=%e7%82%8e%e7%97%87%e5%9b%a0%e5%ad%90&tjType=sentence&style=&t=inflammatory+cytokines"inflammatorycytokinestoHYPERLINK"http://dict.cnki.net/dict_result.aspx?searchword=%e6%94%b9%e5%96%84%e8%83%b0%e5%b2%9b%e7%b4%a0%e6%8a%b5%e6%8a%97&tjType=sentence&style=&t=improve+insulin+resistance"improveinsulinresistance.
【Keywords】Ti