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P40假基因.pdf

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VeterinaryMicrobiology104(2004)213–217 www.elsevier.com/locate/vetmic Shortcommunication Thep40*adhesinpseudogeneofMycoplasmabovis AnneThomasa,1,AnnickLindena,JacquesMainila,IsabelleDiziera, JoelB.Basemanb,ThirumalaiR.Kannanb,Be´ne´dicteFleuryc, JoachimFreyc,EdyM.Vileic,* aDepartmentofInfectiousandParasiticDiseases,FacultyofVeterinaryMedicine,UniversityofLie`ge, B43A,SartTilman,4000Lie`ge,Belgium bDepartmentofMicrobiologyandImmunology,UniversityofTexasHealthScienceCenteratSanAntonio, SanAntonio,TX78229,USA cInstituteofVeterinaryBacteriology,UniversityofBern,La¨nggass-Strasse122,Postfach,3001Bern,Switzerland Received6April2004;receivedinrevisedform2September2004;accepted16September2004 Abstract Ananalogueoftheadhesingenep40ofMycoplasmaagalactiaewasfoundinMycoplasmabovis.Nucleotidesequence analysisofthep40*geneinM.bovisrevealedthepresenceofalargedeletioninvolvingaframeshiftthatcausespremature truncationofthetranslatedprotein,indicatingthatp40*existsasapseudogeneinM.bovis. #2004ElsevierB.V.Allrightsreserved. Keywords:Mycoplasmabovis;p40*gene;Pseudogene;Evolution;Adhesin 1.Introduction(variablesurfaceproteins)areinvolvedincytadher- enceofM.bovis(Sachseetal.,1996,2000).Recent Mycoplasmabovisisthemostimportantmyco-resultssuggestthatotherproteinscouldalsobe plasmaspeciesincattleincountriesfreeofcontagiousimplicatedinthefirststepofinfection(Thomasetal., bovinepleuropneumonia(Burnensetal.,1999;Brice2003).M.bovis(previouslycalledMycoplasma etal.,2000;Kusilukaetal.,2000;Byrneetal.,2001;agalactiaesubsp.bovis)wasdifferentiatedfrom Thomasetal.,2002).AdherencetohostcellsisaMycoplasmaagalactiaeby16SrRNAanduvrC prerequisiteforcolonizationandinfection(Razinetgenesanalysis(Mattssonetal.,1994;Subramaniamet al.,1998).SeveralproteinssuchasP26andVspsal.,1998;Thomasetal.,2004).However,thesetwo mycoplasmaspeciesshareseveralantigens(Rasberry *Correspondingauthor.Tel.:+41316312369;andRosenbusch,1995)andgenessuchasvspA fax:+41316312634.(Flitman-Teneetal.,1997,2000)andthetyrosine– E-mail