预览加载中，请您耐心等待几秒...

内皮祖细胞的研究进展2008.ppt

预览
1/10
2/10
3/10
4/10
5/10
6/10
7/10
8/10
9/10
10/10

亲，该文档总共48页，到这已经超出免费预览范围，如果喜欢就直接下载吧～

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

Definition Theyarecirculating,bonemarrow-derivedcellsthatarefunctionallyandphenotypicallydistinctfrommatureendothelialcells Theycandifferentiateintoendothelialcellsinvitro,asassessedbyexpressionprofilesandfunctionalcharacteristics Theycancontributetoinvivovasculogenesisand/orvascularhomeostasisEmbryonicEPCAdultEPCOriginanddifferentiationofendothelialprogenitorcellsEPCs’DevelopmentVEGFRandVEGF Receptor VEGFR-1(flt-1) VEGFR-2(flk-1/KDR) VEGFR-3(flt-4) Ligand VEGF、VEGF-B,C,D,EVEGFRandVEGFTiereceptorandligand：受体酪氨酸激酶家族 receptor Tie-1(Tie) Tie-2(Tek) Tie-2ligand：Angiopoietin(Ang，血管生成素) Ang-1：血管内皮化 Ang-2：血管周围细胞和内皮细胞分离Tiereceptorandligand：受体酪氨酸激酶家族 Tie-2缺陷的胚胎不能建立血管结构的完整性 Ang-1缺陷鼠也表现为血管生成缺陷 Ephsfamily Erythropoietinproducinghepatocytereceptor(Eph) Ligand:ephrins(EphA、B) EphB可调节Ang-1和Tie-2的表达CD34+,Flk-1+CD34-selectedculture-dishnon-adherentputativeEPC(CDNAC)changetoamorematureendothelialphenotypeduringdifferentiationwhiletheprogenitorphenotyperemainsstableandthemonocyticphenotypedecreases.CD34-selectedputativeEPCfromculture-dishadherentcells(CDAC)expresslowerlevelsofendothelialandprogenitormarkersthanCDNAC.VEGFR-2/Flk-1+CD34+/VEGFR-2+cellscanbehaveasEPCs. CD133+/CD34+/VEGFR-2+cellsrepresentamoreprimitiveEPC.PutativeEPCstakeupacetylatedlowdensitylipoprotein(Ac-LDL)andbindtheendothelialspecificlectinUEA-1.MobilizationofEPCsMobilizationofEPCsChemotaxis,MigrationandInvasion SDF-1 VEGFEPCswereoriginallythoughttobepresent onlyduringembryonicdevelopment. Evidenceaccumulatingoverseveralyears suggeststhattheycanpersistinadultlife. Thishasgeneratedinterestintheuseof EPCsforneovascularizationofischemicor injuredtissueandfortheclinical assessmentofriskfactorsforvarious diseases. EPCAngiogenesis VasculogenesisAngiogenesis aregulatedprocessinvolvingtheproliferation,andmigrationofendothelialcellsfromadjacentpre-existingbloodvessels.Vasculogenesis aregulatedprocessfollowingdifferentiationofendothelialprogenitorcellsfrommesodermalprecursors/endothelialprogenitorcells(EPCs)Vasculogenesis和An