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外源性血管内皮祖细胞在裸鼠肺癌肿瘤模型的筛选模式.pdf

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ChineseJournalofCancer 窑OriginalArticle窑 Therecruitmentofexogenousendothelialprogenitor cellsinlungtumormodelofnudemice QiangPeng1,MingLiu1,Shu­MinSong1,Xian­HuaLi2,Yi­HuaDu1,YongZhi1,Min­YongWang3 1DepartmentofSurgery,AffiliatedHospitalofLuzhouMedicalCollege,Luzhou,Sichuan646000,P.R.China;2Physiology Laboratory,LuzhouMedicalCollege,Luzhou,Sichuan646000,P.R.China;3CentralLaboratory,LuzhouMedicalCollege, Luzhou,Sichuan646000,P.R.China AbstractBackgroundandObjective: 揖铱 Methods: Results: Conclusions: Keywords: Endothelialprogenitorcells(EPCs)playanimportantexogenousEPCstotumor,thisstudyestablishedahuman roleinthegrowthoftumor.However,whethercirculatinglungadenocarcinomamodelinnudemicetoinvestigatethe EPCsparticipateintumorvasculogenesisremainrecruitmentofEPCs. controversial.Somestudies[1­3]showthat,althoughcirculating vascularEPCscanberecruitedtothetumorvesselwall,it doesnotconstituteapartoftumorvesselandhasno contributiontotumorgrowth.Otherstudies[4,5]showedthat exogenousEPCsnotonlyparticipateinangiogenesisof tumorbutalsohavesignificantinfluenceonthegrowthof tumor.Manycytokinessuchashypoxia­induciblefactorTheprotocolwasapprovedbytheAffiliatedHospitalof (HIF),vascularendothelialgrowthfactor(VEGF),andLuzhouMedicalCollege.Informedconsentwasobtained stromalcell­derivedfactor(SDF)playcrucialrolesinthefrom3donors.Allofthemwerehealthypregnantwomen recruitmentofEPCs.TounderstandtheactionofadministratedbyCaesareanbirth,agefrom22to26. Humanumbilicalcordbloodsample(50mL)wascollected Correspondenceto:QiangPeng;Tel:+86­830­3165431;fromfreshumbilicalcordwithplacenta.Aftercentrifugation Fax:+86­830­3165437;Email:pengqiangpq@sina.com.cn(280×for10min),thecellpelletswereresuspendedin5 ThispaperwastranslatedfromChineseintoEnglishbyMedicalmLDulbeccosmodifiedEaglesmedium(DMEM,Sigma, TranslationandeditedbyWeiLiu.爷爷 Received:2010­04­14;Accepted:2010­09­14USA)containing10%fetalcalfserum(FCS,Sigma,USA), Grants:ChineseNationalNaturalScienceFoundationProgram(No.thenaddedtothetopo