大黄高效液相色谱法指纹图谱分离条件的优化.pdf

四川大学学报(医学版)
2004;35(4):559562
JSichuanUniv(MedSciEdi)

大黄高效液相色谱法指纹图谱分离条件的优化3

徐雄良,张志荣△,黄园
四川大学华西药学院靶向药物与新型给药系统重点实验室(成都610041)

【摘要】目的优化大黄高效液相色谱法指纹图谱(HPLC2FPs)的分离条件。方法以RP2HPLC法,使用
Shim2packCLC2ODS柱(6.0mmi.d.×150mm,5Lm),流动相为甲醇21mlöL磷酸溶液,检测波长为254nm,柱温
为40℃,考察大黄总提取物在不同梯度洗脱条件下的分离情况。结果优选出的大黄HPLC2FPs分离条件,可使
大黄总提取物中各组分达到较佳分离,稳定性、精密度、重现性好。结论该色谱分离条件可用于建立大黄药材
HPLC2FPs的分离条件。
【关键词】大黄高效液相色谱法指纹图谱分离条件
【中图分类号】R92,O65,R282

OptimizationoftheSeparationConditionsofFingerprintforRhubarbbyHighPerformanceLiquid
△
ChromatographyXUXiong2liang,ZHANGZhi2rong,HUANGYuan.KeyLaboratoryofTargetingandNew
DrugDeliverySystems,WestChinaSchoolofPharmacy,SichuanUniversity,Chengdu610041,China
【Abstract】ObjectiveTooptimizetheseparationconditionsoffingerprintforRhubarbbyhighperformance
liquidchromatographyandlaythefoundationofstudiesonitsfingerprintbyhighperformanceliquid
chromatography.MethodsThereversed2phasehighperformanceliquidchromatographicmethodwasadopted.
ThesampleswereextractedbyultrasoundandseparatedbyalineargradientelutiononShim2packCLC2ODS
column(6.0mmi.d.×150mm,5Lm),andthen,withthemethanol21mlöLphosphoricacidsolutionasmobile
phase,weredetectedatUV254nmandcolumntemperature40℃.Duringthelineargradientelutionprogram,
thevolumefractionofmethanolinmobilephasechangedasfollows:0min25min,20%;5min225min,from20%
to50%;25min235min,from50%to80%;35min260min,from80%to90%;60min270min,90%.Theflow
ratewas0.8mlöminfrom0minto35min,0.6mlöminfrom35minto50minand1.0mlöminafter50min.
ResultsTheseparationconditionsdescribedaboveachievedgoodresultintheaspectsofseparableeffect,
stability,sensitivity,andreproducibility.ConclusionTheoptimumseparationconditionsoffingerprintwouldbe
usefulforestablishingthemethodoffingerprintforRhubarbbyhighperformanceliquidchromatography.
【Keywords】RhubarbHighperformanceliquidchromatographyFingerprintSeparation
conditionsofchromatogram

以高效液相色谱法指纹图谱(HPLC2FPs)来评纹峰,优选大黄HPLC2FPs的分离条件,探讨大黄
价中药材及其制剂的质量已成为我国中药研究的热蒽醌类衍生物在甲醇2水二元溶剂体系中的色谱分
点之一,但优选适宜的分离条件一直是色谱分析和离行为。
中药材及其制剂分析中最困难的步骤之一。因此,探
1材料与方法
求色谱保