利用SRAP标记构建甘薯分子连锁图谱.pdf

作物学报ACTAAGRONOMICASINICA2010,36(8):1286−1295http://www.chinacrops.org/zwxb/
ISSN0496-3490;CODENTSHPA9E-mail:[e-mail]

DOI:10.3724/SP.J.1006.2010.01286

利用SRAP标记构建甘薯分子连锁图谱

1,21,*2213
李爱贤刘庆昌王庆美张立明翟红刘树震

1中国农业大学农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利
用重点实验室,北京100193;2山东省农业科学院作物研究所,山东济南250100;3山东省济南市农业局,山东济南250021

摘要:以高淀粉甘薯品种漯徐薯8号为母本,低淀粉甘薯品种郑薯20为父本,杂交得到的F1分离群体的240个单
株为作图群体,利用SRAP标记技术,共得到770个母本的多态性标记和523个父本的多态性标记,用JoinMap3.0
软件和“双假测交”策略,分别构建了2个亲本的分子连锁图谱。其中漯徐薯8号的图谱包括由473个SRAP标记组
成的81个连锁群,总图距为5802.46cM,标记间平均图距为10.16cM;郑薯20的图谱包括由328个SRAP标记组
成的66个连锁群,总图距为3967.90cM,标记间平均图距为12.02cM。该高密度分子连锁图谱的构建,为甘薯分子
标记辅助选择、基因定位及克隆研究奠定了基础。
关键词:甘薯;分子连锁图谱;SRAP标记

EstablishmentofMolecularLinkageMapsUsingSRAPMarkersinSweetPotato

LIAi-Xian1,2,LIUQing-Chang1,*,WANGQing-Mei2,ZHANGLi-Ming2,ZHAIHong1,andLIUShu-Zhen3

1KeyLaboratoryofCropGenomicsandGeneticImprovement,MinistryofAgriculture/BeijingKeyLaboratoryofCropGeneticImprovement/Key
LaboratoryofCropHeterosisandUtilization,MinistryofEducation,ChinaAgriculturalUniversity,Beijing100193,China;2CropResearchInstitute,
ShandongAcademyofAgriculturalSciences,Jinan250100,China;3JinanAgriculturalBureau,Jinan250021,China

Abstract:Sweetpotato[Ipomoeabatatas(L.)Lam.]isanimportantvegetativereproductioncrop.Despiteitsworldwideimpor-
tance,molecularbiologyresearchesinsweetpotatohavelaggedbehindothercropsduetoitscharacteristicsincludingpolyploidy,
outcrossingbehavior,andhighheterozygosity.Asaresult,itisdifficulttomakeabreakthroughfortheimprovementofsweet
potatousingconventionalmethods.Inthisstudy,sequence-relatedamplifiedpolymorphism(SRAP)markerswereappliedtoes-

tablishmoleculargeneticlinkagemapswithF1populationconsistingof240individualsderivedfromacrossofsweetpotatocv.
Luoxushu8(highstarchcontentcultivar)×cv.Zhengshu20(lowstarchcontentcultivar).Atotalof770and523SRAPmarkers
weregenerated,respectively,inLuoxushu8andZhengshu20.UsingthesoftwareofJoinMap3.0andthe‘doublepseudotest-
crossstrategy’,amolecularlinkagemapcontaining81linkagegrou