相关凋亡诱导配体TRAIL及其受体在急性髓系白血病细.pdf

中国实验血液学杂志JournalofExperimentalHematology2005;13(1):65-69·56·

文章编号(ArticleID):1009-2137(2005)01-0065-05·论著·

肿瘤坏死因子相关凋亡诱导配体TRAIL及其受体在
急性髓系白血病细胞中的表达及意义

赵氵是,王红祥1,毛红,肖娟1,邹萍1
武汉市中心医院血液科,武汉430014,1华中科技大学同济医学院血液病研究所,武汉430022

摘要为了检测肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体在急性髓系白血病细胞中的表达,并探讨其在白血
病治疗中的意义,采用RT2PCR方法及流式细胞术,对39例急性髓系白血病细胞(患者组)、18例完全缓解白血病细胞(缓
解组)和21例正常人骨髓或外周血单个核细胞(对照组)表面TRAIL及其受体的表达进行检测。结果表明:①患者组和
缓解组TRAIL、DR4和DR5表达高,而DcR1和DcR2表达低。②缓解组DR5的表达高于患者组。③患者组和缓解组
DR5的表达均高于DR4。④患者组AML不同亚型表达TRAIL及其受体相似。结论:TRAIL及其受体在急性髓系白血
病细胞中的表达具有明显的差异性。DR5在TRAIL介导的急性髓系白血病细胞凋亡中起重要的作用。
关键词肿瘤坏死因子相关凋亡诱导配体;急性髓系白血病;基因表达
中图分类号R733171文献标识码A

ExpressionandItsSignificanceofTRAILandItsReceptorsinCellsofPa2
tientswithAcuteMyeloidLeukemia
ZHAOShi,WANGHong2Xiang,MAOHong,XIAOJuan,ZOUPing
DepartmentofHematology,WuhanCentralHospital,Wuhan430014,China;1InstituteofHematology,TongjiMedicalCollege,
HuazhongUniversityofScienceandTechnology,Wuhan430022,China
AbstractThisstudywasaimedtodetecttheexpressionofTNFrelatedapoptosis2inducingligand(TRAIL)anditsre2
ceptorsonacutemyeloidleukemic(AML)cells,andexploreitspossibleroleinleukemiatherapy.RT2PCRandflowcy2
tometrywereusedtodetecttheexpressionofTRAILanditsreceptorsonAMLcellsof39cases(patientgroup),AML
cellsof18caseswithcompleteremission(CRgroup)andBMMNCorPBMNCof21normalpersons(controlgroup).
Theresultsshowedthat(1)TRAIL,DR4andDR5werehighlyexpressedinbothpatientgroupandCRgroup,whilethe
DcR1andDcR2werepoorlyexpressed.(2)ThelevelofDR5expressioninCRgroupwashigherthanthatinpatient
group.(3)ThelevelofDR5washigherthanDR4inbothpatientgroupandCRgroup.(4)TRAILanditsreceptors
wereexpressedsimilarlyindifferentsubtypesofAML.Inconclusion,therearedifferencesbetweentheexpressionsof
TRAILanditsreceptorsinAMLcells.DR5mayplayanimportantroleinTRAIL2inducingapoptosisofAMLcells.
Keywordstumornecrosisfactor2relatedapoptosis2inducingligand;acutemyeloidleukemia;geneexpression
JExpHematol2005;13(1):65-69

肿瘤坏死因子相关凋亡诱导配体(TNFrelatedap2诊断标准见《血液病诊断及疗效标准》1。男23例,

optosisinducingligand,TRAIL)是新近发现的肿瘤女16例,中位年龄