花生栽培种SSR遗传图谱的构建.pdf

作物学报ACTAAGRONOMICASINICA2009,35(3):395−402http://www.chinacrops.org/zwxb/
ISSN0496-3490;CODENTSHPA9E-mail:[e-mail]

DOI:10.3724/SP.J.1006.2009.00395

花生栽培种SSR遗传图谱的构建

*
洪彦彬梁炫强陈小平刘海燕周桂元李少雄温世杰

广东省农业科学院作物研究所,广东广州510640

摘要:花生栽培种品种间分子多态性相对缺乏,至今未构建出较完整的分子遗传图谱。本研究以粤油13和阜95-5

为亲本,通过杂交构建包含184个F6重组自交系的遗传作图群体。采用652对genomic-SSR引物和392对EST-SSR
引物对亲本进行多态性检测,从中筛选出121对多态性引物,在亲本中共检测到123个多态性位点。利用作图群体对
多态性SSR位点进行遗传连锁分析,获得包含108个SSR标记(102个genomic-SSR标记和6个EST-SSR标记),涉
及20个连锁群,总长568cM,平均图距为6.45cM的花生栽培种遗传图谱。与前人构建的花生野生种(A.duranensis×
A.stenosperma,AAgenome)SSR遗传图谱比较,初步确定本研究构建的遗传图谱中有11个连锁群与野生种遗传图谱
的6个连锁群存在同源关系。
关键词:花生;SSR;遗传图谱

ConstructionofGeneticLinkageMapinPeanut(ArachishypogaeaL.)Culti-
vars

HONGYan-Bin,LIANGXuan-Qiang*,CHENXiao-Ping,LIUHai-Yan,ZHOUGui-Yuan,LIShao-Xiong,
andWENShi-Jie

CropsResearchInstitute,GuangdongAcademyofAgriculturalSciences,Guangzhou510640,China

Abstract:Moleculargeneticmapisafundamentalorganizationaltoolforgenomicresearch.However,acomprehensivegenetic
linkagemapforpeanutcultivarshasnotyetbeendevelopedduetoextremelylowlevelsofDNApolymorphismsincultivated
peanut.Inthisstudy,184recombinantinbredlines(RIL),derivedfromacrossbetweentwoSpanishtypepeanutcultivars
(Yueyou13×Fu95-5),wereusedasmappingpopulation.Atotalof652pairsofgenomic-SSRprimersand392pairsofEST-SSR
primerswereusedtodetectthepolymorphismsbetweentheparentalcultivars.Ofthem,121SSRprimerpairsamplified123seg-
regatinglociandwereselectedtoanalyzetheRILpopulation.Ageneticlinkagemapconsistingof108SSRloci(102ge-
nomic-SSRand6EST-SSR)in20linkagegroupsandcovers568cMwithanaveragedistanceof6.45cMofintermakerwascon-
structed.BycomparingtheSSRgeneticmapofArachis(A.duranensis×A.stenosperma)withAAgenome,11linkagegroupsin
thelinkagemapconstructedinthisstudywereconfirmedtohavehomologywith6linkagegroupsofwildpeanutspecies.
Keywords:Peanut(ArachishypogaeaL.);SSR;Geneticlinkagemap

分子遗传图谱是植物基因组结构分析和比较的栽培种间缺乏多态性[1-3],致使遗传图谱构建进展缓
有力工具,已被广泛应用于基因定位、图位克隆、慢。基于上述标记构建的花生栽培种遗传图谱鲜见
比较基因组学和分子标记辅助育种等研究。花生栽报道。虽然Herselma