醛糖还原酶基因多态性及其活性与2型糖尿病微血管病变的.pdf

Interrelationshipamongthepolymorphismof(AC)ninthe
i;，-endoftheARgene，theerythrocytealdosereductase
activityandsusceptibilityofmicroangiopathyinthepatients
withtype2diabetesmellitus

Abstract

ObjectiveTostudytheinterrelationshipamongthepolymorphismof(AC)ninthe
5'-endoftheARgene,theerythrocytealdosereductaseactivityandsi!sceptibility

ofmicroangiopathyinthepatientswithtype2diabetesmellitus(DM).Methods

Onehundredandfortycasesoftype2DMandforty-ninecontrolswereincluded
inthisstudy.AllthesecasesweretheHannationalityofGuangxizhuang

autonomousregion.ThesubjectsweredevidedintoDMwithoutdiabetic

microangiopathy(NDC)(56cases),DMAPgrouy(84cases),andnormal

controls(CON)(44cases),furthermore,accordingtothetypeofallele,thesubjects

weredevidedintoZ+2,Z-2,bothZ+2andZ-2,neitherZ+2norZ-2(X/X).PCR

wasperformedandPCRproductswereelectrophoresedwith8%formamid/urea

gel.ARactivity(ARA)wasmeasuredbymodifiedSriratavamethod.The

differencesofARAwerecomparedamongthesegroups.ResultsItshowed7types
ofalleles(Z-6-Z+6).ThefrequencyofalleleZ+2wasmuchlowerinDMgroup

thaninNDCgroupandCONgroup.ARAinDMAPgroupwassignificantlyhigher

thanthatinNDCgroup,ARAinNDCgroupwassignificantlyhigherthanthatin

CONgroup.AmongZ-2,Z+2,Z+2/Z-2andX/Xgroups.ARAwasthehighestin

DMZ-2/Z-2groupandthelowestinDMZ十21Z+2group.ConclusionZ-2

.1。
allelemightbethesusceptiblegeneandZ+2allelemightbetheprotectedgene.

TheactivationofARplaysanimportantroleinthedevelopmentofDMAEZ-2

allelemaybeanactivatorandallelemaybeaninhititorofAR
IKeywords]aldosereductase;polymorphism;type2diabetesmellitus;diatetic

complication;

-2-
醛糖还原酶基因多态性及其活性与
2型糖尿病微血管病变的相关性研究

摘要

目的研究醛糖还原酶(AR)基因5’端(AC)n的多态性及其活性与2型糖尿
病(DM)微血管病变的关系。方法采用聚合酶链反应(PCR)，检测140例2
型糖尿病患者，其中无微血管病变组(NDC)56例和微血管病变组(DMAP)94
例(糖尿病肾病组(DN)30例，糖尿病视网膜病变组(DRP)24例，糖尿病
神经病变组(DPN)30例)和49例正常对照组(CON),AR基因5‘端(AC)n

的多态性，同时用改良Sriratava法测定AR活性并比较其在各组间的差异。
结果①DM组和CON组均发现7种等位基因(Z-6^-Z+6),Z+2等位基因频率

在PKAP组显著低于NDC和CON组(P<0.05).②DMAP组、NDC组和CON三组

间的AR活性(ARA)差异有显著性，DMAP组最高，NDC组次之，CON组最低
(P<0.001).DM