利用SRAP标记构建18个木薯品种的DNA指纹图谱.pdf

作物学报ACTAAGRONOMICASINICA2010,36(10):1642−1648http://www.chinacrops.org/zwxb/
ISSN0496-3490;CODENTSHPA9E-mail:[e-mail]

DOI:10.3724/SP.J.1006.2010.01642

利用SRAP标记构建18个木薯品种的DNA指纹图谱

1,23111,*
齐兰王文泉张振文叶剑秋李开绵

1中国热带农业科学院热带作物品种资源研究所,海南儋州571737;2海南大学农学院,海南儋州571737;3中国热带农业科学院热
带生物技术研究所,海南海口571101

摘要:利用SRAP标记,选用36对多态性较好的引物组合,对18个木薯品种进行分析鉴定,扩增出320个位点,其
中多态性位点235个,多态性比率达73.4%,平均每对引物组合可产生8.9个位点和6.5个多态性位点;235个多态性
位点采用非加权组平均法(UPGMA)进行聚类分析,以遗传相似系数0.734为阈值,可将18份供试材料分为4组;选
用2对多态性引物Me1-Em5和Me24-Em10,初步构建了18份木薯品种的指纹图谱,根据条带的有无转换为0、1二
进制编码形成数字指纹,每个品种拥有唯一的数字指纹区别于其他品种,置信概率达到99.999%。结果表明,采用
SRAP标记建立的指纹图谱适用于木薯品种的分类和鉴定。
关键词:木薯;SRAP;DNA指纹图谱

DNAFingerprintingAnalysisof18CassavaVarietiesUsingSequence-Related
AmplifiedPolymorphismMarkers

QILan1,2,WANGWen-Quan3,ZHANGZhen-Wen1,YEJian-Qiu1,andLIKai-Mian1,*

1TropicalCropsGeneticResourcesInstitute,ChineseAcademyofTropicalAgriculturalSciences,Danzhou571737,China;2HainanUniversity,
Danzhou571737,China;3InstituteofTropicalBioscienceandBiotechnology,ChineseAcademyofTropicalAgriculturalSciences,Haikou571101,
China

Abstract:Therewidelyexistsacaseofsamenameforsomevarietiesordifferentnamesforonevarietyincassavaagriculture
production,soitisdifficulttoidentifycassavavarietiesonlyfromthephenotypiccharacteristics.Inthisstudy,SRAPmarkerwas
appliedtodetect18cassavavarieties,thirty-sixprimercombinationswereselectedwithabundantpolymorphism,atotalof320
bandswerescored,235(73.4%)outofthemwerepolymorphic,withanaverageof8.9bandsand6.5polymorphicbandsforeach
primercombination,235polymorphicbandswereusedtodevelopdendrogramwithUnweightedPair-GroupMethodArithmetic
Average(UPGMA),and18cassavavarietiesweredividedintofourmajorgroupsatthe0.734similaritylevel;twoprimercombi-
nationsMe1-Em5andMe24-Em10,wereusedtodeveloptheDNAfingerprintsforthe18cassavavarieties.TheDNAfingerprints
werethenconvertedintobinarycodes,with1and0representingbandpresenceandabsence,respectively.IntheDNAfingerprints,
eachofthe18cassavavarietieshaditsuniquebinarycode,whichdistinguishedv