肝内胆管结石并肝内胆管癌.doc

肝内胆管结石并肝内胆管癌组织中HSP27和Caspase-9的表达及与凋亡的关系
【摘要】目的：探讨肝内胆管结石并肝内胆管癌组织中HSP27和Caspase-9的表达及与凋
亡的关系。方法：选取我院于2010年10月至2013年10月间收集的病例资料和手术切除标本60例作为研究对象，并将其分为三组，A组正常胆管组12例，B组肝内胆管结石组28例，C组肝内胆管结石并肝内胆管癌组20例。通过免疫组织化学法分别对三组的HSP27与Caspase-9的表达情况进行检测，并研究分析HSP27和Caspase-9与细胞凋亡之间的关系。结果：三组标本的HSP27和Caspase-9的表达阳性率分别为0、32.14%（9/28）和75.00%（15/20），C组明显高于其他两组(P<0.05)，肝内胆管结石并肝内胆管癌组织中，HSP27和Caspase-9的表达呈负相关关系（r=-0.378，P=0.020）；三组的凋亡指数（apoptosisindex，AI）分别为12.65±2.16%、14.26±3.77%、3.68±2.71%，A、B两组的AI值明显高于C组P＜0.05）结论：HSP27的高表达或Caspase-9的低表达都更容易造成淋巴结转移或门静脉浸润的现象，Hsp27能够通过抑制Caspase-9的活性从而抑制细胞凋亡。
【关键词】肝内胆管结石；肝内胆管癌组织；表达与凋亡
ExpressionofHSP27andCaspase-9anditsrelationshiptoapoptosisinhepatolithiasisassociatedintrahepatic
[Abstract]Objective:ToinvestigatetheexpressionofHSP27andCaspase-9anditsrelationshiptoapoptosisinhepatolithiasisassociatedintrahepatic.Methods:Theclinicaldataof60casesbetweenOctober2010toOctober2013wasanalyzedinourhospital,andthe60casesweredividedintothreegroups,Agroup:normalbileductgroup(12cases),groupB:livergroupwithbileductstones(28cases),groupC:groupwithintrahepaticbileductstonesintrahepaticcholangiocarcinoma(20cases).HSP27expressionandCaspase-9ofthreegroupsweredetectedbyimmunohistochemistry,respectively,andtostudyandanalyzetherelationshipbetweenHSP27andapoptosisCaspase-9between.Results:HSP27andpositiveexpressionrateofthethreegroupsofspecimensCaspase-9was0,32.14%(9/28)and75.00%(15/20),respectively,groupCwassignificantlyhigherthantheothertwogroups(P<0.05).TheexpressionofHSP27andCaspase-9wasnegativelycorrelatedwithliverbileductstonesandintrahepaticcholangiocarcinomatissues,(r=-0.378,P=0.020);threegroupsofapoptoticindex(apoptosisindex,AI)was12.65±2.16%,14.26±3.77%,3.68±2.71%,AIvalueofgroupAandBwassignificantlyhigherthangroupCP<0.05)Conclusion:ThelowexpressionofHSP27expressionorCaspase-9aremorelikelytocauselymphnodemetastasisorportalveininfiltrationphenomenon,HSP27caninhibittheactivityofCaspase-9toinhibitapoptosis.
Keywords:intrahepaticbileductstones;intrahepaticcholangiocarcinoma;expres