陆地棉遗传图谱构建及产量和纤维品质性状QTL定位.pdf

作物学报ACTAAGRONOMICASINICA2008,34(7):1199−1205http://www.chinacrops.org/zwxb/
ISSN0496-3490;CODENTSHPA9E-mail:[e-mail]

DOI:10.3724/SP.J.1006.2008.01199

陆地棉遗传图谱构建及产量和纤维品质性状QTL定位

*
陈利张正圣胡美纯王威张建刘大军郑靓郑风敏

马靖

(西南大学农学与生物科技学院/农业部生物技术与作物品质改良重点实验室,重庆400716)

摘要:利用3458对SSR引物筛选陆地棉中棉所35和渝棉1号间的多态性引物,获得173对。以多态性引物检测

(渝棉1号×中棉所35)F2群体180个单株的标记基因型,共获得178个标记位点。构建的遗传连锁图谱包括148个标
记,36个连锁群,总长1309.2cM,标记间平均距离8.8cM,覆盖棉花基因组的29.5%。36个连锁群中的28个分别

被定位于20条染色体,8个连锁群未定位于染色体。以渝棉1号×中棉所35的F2、F2:3群体的产量、纤维品质性状鉴
定结果,利用区间作图方法,检测到4个产量性状QTL,即2个衣分(LP)、1个铃重(BW)、1个籽指(SD);5个纤维品
质性状QTL,即1个纤维长度(FL)、2个纤维比强度(FS)和2个纤维细度(FF)。LP1、BW、SD、FL和FS1被定位于
第7染色体,LP2、FS2、FF1和FF2被分别位于第15、21、9和20染色体。5个纤维品质QTL的有利等位基因均来
源于渝棉1号。
关键词:陆地棉;遗传连锁图谱;产量;纤维品质;QTL

GeneticLinkageMapConstructionandQTLMappingforYieldandFi-
berQualityinUplandCotton(GossypiumhirsutumL.)

CHENLi,ZHANGZheng-Sheng*,HUMei-Chun,WANGWei,ZHANGJian,LIUDa-Jun,ZHENGJing,
ZHENGFeng-Min,andMAJing

(KeyLaboratoryofBiotechnology&CropQualityImprovement,MinistryofAgriculture/CollegeofAgronomy&Biotechnology,SouthwestUni-
versity,Chongqing400716,China)

Abstract:Cottonistheworld’smostimportantnaturaltextilefiber.Withthechangesinspinningtechnology,theimprovement
ofcottonfiberqualityisbecomingextremelyimportant.ThecombinationofconventionalbreedingtechniqueswithMAS(mo-
lecular-assistedselection)willbecomeabasicapproachforcottonfiberimprovement.Inthepresentstudy,3458SSRprimer
pairswereusedtoscreenthepolymorphicprimersbetweenZhong35andYumian1,and173polymorphicprimerpairswereob-

tained.Thepolymorphicprimerpairswereusedtogenotypethe180individualplantsofF2populationderivedfrom(Zhong35×
Yumian1),and178lociwereobtained.Ageneticlinkagemapwith148SSRlociand36linkagegroupswasconstructed,andthe
linkagemapcoveredawholelengthof1309.2cMwithanaverageintervalof8.8cMbetweentwomarkers,accountingfor
29.5%ofthecottongenome.28outof36linkagegroupswerelocatedon20chromosomes,and8linkagegroupswerenotlocated

onanychromosomes.Thedataofyieldandfiberqualitiesofthe180F2plantsandF2:3familyli