噬菌体抗体库技术及其在水产养殖的应用前景.pdf

第28卷第3期水产学报Vol.28,No.3

2004年6月JOURNALOFFISHERIESOFCHINAJune,2004

文章编号:1000-0615(2004)03-0329-05
综述

噬菌体抗体库技术及其在水产养殖的应用前景

章晋勇,吴英松,汪建国
(中国科学院水生生物研究所,湖北武汉430072)

关键词:噬菌体抗体库;应用;前景;水产养殖
中图分类号:Q782;S917文献标识码:A

Progressofphageantibodylibrarytechniqueand
itsapplicationprospectinaquaculture

ZHANGJinyong,WUYingsong,WANGJianguo
(InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China)

Abstract:Phagedisplayantibodylibraryhasbeenproventobeapowerfultechniqueusedindevelopmentof
antibodies.Sinceitwasestablishedin1990,thetechnologyhasmadeenormousimprovementandplayedmoreand
moreimportantroleinbasicresearchofbiology,immunology,oncology,proteinengineering,ligandreceptor
studiesandproteomicsamongothersinlasttwodecadeswhilethereisnoreportabouttheapplicationofitin
aquaculturesofar.Itisasuccessimplicationofphagedisplaytechniqueinantibodyengineeringinwhichantibodies
orantibodyfragmentsaredisplayedonthesurfaceoffilamentousbacteriophagebygeneticfusiontoacoatproteinof
phage.Cooperatingwiththeeffectivescreeningtechnique,affinitypanning,theseformtheprincipleofphage
displayantibodylibrary.Themostcharacteristicofitisadirectphysicallinkbetweenphenotypeandgenotype.So,
thetechnologymakesitpracticabletoimprovecharacteristicofselectedantibodiesbygeneticmanipulation.Inthe
presentwork,thebackground,principleandadvantagesofthepowerfultoolovertraditionalhybridromatechnolgy
aresummarized.Inaddition,severalkeyproblemspossibletofaceinthecourseofapplicationofthetechnology,
includingimprovingthediversityoflibrary,augmentationoflibrarysize,generationofhighaffinityantibodyand
effectivescreeningofspecificantibodyweredissertated.Atlastthepossibleimplicationprospectofphagedisplay
antibodytechniqueinaquaculturewasdiscussed,especiallyinelucidatingtheimmunesystemoffishandproducing
largeamountantibodieswithimportantdiagnosticandtherapeuticvalueinfishdiseases.
Keywords:phageantibodylibrary;application;prospect;aquaculture

近十多年来,随着分子生物学(包括基因工程、蛋白质抗体技术已从细胞工程抗体(