土鸡肠道产消化酶细菌的研究.doc

12株信鸽分离菌产脂肪酶初步研究Page0Page0Page0Page0Page0Page0Page0Page0Page1本科生毕业论文(设计)题目：土鸡肠道产消化酶细菌的研究学生姓名：陈旭斌学号：201013010310专业班级：生科10103指导教师：李娜完成时间：2014年4月30日目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc449473533"摘要PAGEREF_Toc449473533\h1HYPERLINK\l"_Toc449473534"AbstractPAGEREF_Toc449473534\h1HYPERLINK\l"_Toc449473535"1绪论PAGEREF_Toc449473535\h2HYPERLINK\l"_Toc449473536"1.1信鸽PAGEREF_Toc449473536\h2HYPERLINK\l"_Toc449473537"1.2脂肪酶的概述PAGEREF_Toc449473537\h2HYPERLINK\l"_Toc449473538"1.3脂肪酶的研究进展PAGEREF_Toc449473538\h2HYPERLINK\l"_Toc449473539"1.4信鸽分离菌产脂肪酶的研究意义PAGEREF_Toc449473539\h3HYPERLINK\l"_Toc449473540"2实验材料PAGEREF_Toc449473540\h3HYPERLINK\l"_Toc449473541"2.1实验原料PAGEREF_Toc449473541\h3HYPERLINK\l"_Toc449473542"2.2药品和试剂PAGEREF_Toc449473542\h3HYPERLINK\l"_Toc449473543"2.3实验用品PAGEREF_Toc449473543\h3HYPERLINK\l"_Toc449473544"2.4主要设备及仪器PAGEREF_Toc449473544\h3HYPERLINK\l"_Toc449473545"3实验方法PAGEREF_Toc449473545\h4HYPERLINK\l"_Toc449473546"3.1分离计数的过程PAGEREF_Toc449473546\h4HYPERLINK\l"_Toc449473547"3.1.1鸽肝、鸽肺稀释液的制备PAGEREF_Toc449473547\h4HYPERLINK\l"_Toc449473548"3.2菌落特征观察的过程PAGEREF_Toc449473548\h5HYPERLINK\l"_Toc449473549"3.2.1、观察倍比稀释后平板中长出的菌落特征。PAGEREF_Toc449473549\h5HYPERLINK\l"_Toc449473550"3.2.2细菌和酵母菌菌落的观察和识别PAGEREF_Toc449473550\h5HYPERLINK\l"_Toc449473551"3.2.3菌落归类PAGEREF_Toc449473551\h5HYPERLINK\l"_Toc449473552"将记录好的带有编号的未知菌落的各项特征，结合各类微生物特点的，分别了解各菌株的外貌特征并归入相应的大类中。PAGEREF_Toc449473552\h5HYPERLINK\l"_Toc449473553"3.3四区划线的过程PAGEREF_Toc449473553\h6HYPERLINK\l"_Toc449473554"3.3.1、试管斜面划线PAGEREF_Toc449473554\h6HYPERLINK\l"_Toc449473555"3.3.2、平板的四区划线PAGEREF_Toc449473555\h6HYPERLINK\l"_Toc449473556"3.4革兰氏染色的方法PAGEREF_Toc449473556\h7HYPERLINK\l"_Toc449473557"3.5产脂肪酶能力检测的过程PAGEREF_Toc449473557\h8HYPERLINK\l"_Toc449473558"4实验结果PAGEREF_Toc449473558\h8HYPERLINK\l"_Toc449473559"4.1分离计数的结果PAGEREF_Toc449473559\h8HYPERLI