灯盏细辛抑制外源性-VEGF诱导的大鼠视网膜血管病变TGFβ1的表达.docx

灯盏细辛抑制外源性VEGF诱导的大鼠视网膜血管病变TGFβ1的表达【摘要】目的：探讨灯盏细辛(EBHM)对外源性VEGF诱导的大鼠视网膜血管病变眼玻璃体腔TGFβ1和视网膜TGFβ1mRNA表达的影响。方法：大鼠48只随机分为rrVEGF164,rrVEGF164+EBHM,rrVEGF164+NS和正常对照4组。rrVEGF164+EBHM组每天60g/L的灯盏细辛浸膏ip。分别于不同时间收集玻璃体液后采用ELISA检测TGFβ1的浓度,并提取视网膜总的RNA后行RT-PCR检测TGFβ1mRNA的表达。结果：在2wk或6wk时VEGF、VEGF+NS和VEGF+EBHM组实验眼玻璃体内TGFβ1的浓度均高于正常对照组。VEGF+EBHM组低于VEGF组和VEGF+NS组。各组TGFβ1mRNA/GAPDHmRNA的差异与玻璃体内的TGFβ1浓度变化一致。结论：rrVEGF164作用大鼠玻璃体内TGFβ1浓度升高，视网膜TGFβ1mRNA表达增强。灯盏细辛能部分抑制rrVEGF164诱导的大鼠视网膜血管病变玻璃体内TGFβ1浓度的升高和视网膜TGFβ1mRNA表达的增强。【关键词】重组大鼠血管内皮生长因子灯盏细辛转化生长因子β1InhibitionofEBHMontheexpressionofTGFβ1intheretinalvasculopathyinducedbyexogenousVEGFinratsAbstractAIM:ToinvestigatetheeffectsofEBHMonTGFβ1inthevitreousandtheexpressionofretinalTGFβ1mRNAintheretinalvasculopathyinducedbyexogenousVEGFinrats.METHODS:Forty-eightratswererandomlydividedintofourgroups:rrVEGF164,rrVEGF164+NS,rrVEGF164+EBHMandnormalgroup.RatsinrrVEGF164+EBHMgroupwereadministratedintraperitoneallywith60g/LEBHMsolution.CapturedvitreoushumorandtotalextractedretinalRNAwereanalyzedbyELISAfortheconcentrationofTGFβ1andRT-PCRforexpressionofTGFβ1mRNAindifferenttimerespectively.RESULTS:TwoorsixweeksafterthefirstofintravitrealrrVEGF164injection,theconcentrationofTGFβ1inthevitreousofexperimenteyesamongrrVEGF164,rrVEGF164+NSandrrVEGF164+EBHMgroupwerehigherthanthatofinnormalgroup(P).TheconcentrationofTGFβ1inthevitreousofexperimenteyesinrrVEGF164+EBHMgroupwerelowerthanthatofinrrVEGF164andrrVEGF164+NSgroup(P).ThedifferencesintheratioofTGFβ1mRNAandGAPDHmRNAineverygroupwereassameasthatinthechangeofconcentrationofTGFβ1inthevitreous.·CONCLUSION:TGFβ1inthevitreousandexpressionofTGFβ1mRNAincreasedafterintravitrealrrVEGF164haspartialinhibitoryinfluncenceontheelevatedconcentrationofTGFβ1inthevitreousandexpressionofTGFβ1mRNAintheretinalvasculopathyinducedbyexogenousrrVEGF164inrats.·KEYWORDS:rrVEGF164;EBHM;TGFβ10引言血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)是目前已知最重要的血管新生促进因子，而转化生长因子β1(transforminggrowthfactorβ1,TGFβ1)的生理作用比较复杂，对于不同的靶细胞以及同一靶细胞的不同状态下，显示出不同作用。体外实验表明TGFβ1能增