原发性肝癌患者细胞因子水平的变化与转移和复发的关系.docx

原发性肝癌患者细胞因子水平的变化与转移和复发的关系【摘要】目的：观察原发性肝癌患者细胞因子IL4、IFNγ、IL1β在肝癌组织中的表达，探讨细胞因子的变化、漂移与肝癌转移和复发的关系。方法：应用逆转录聚合酶链反应检测并比较肝癌组和对照组细胞因子IFNγ、IL4、IL1β的水平。结果：34例肝细胞性肝癌患者与15例单纯肝硬化患者相比，肝癌组织中IFNγ的表达强度、阳性表达频度较对照组明显降低，分别为±和±。IL4在肝癌组织中的表达强度较对照组明显增强，分别为±和±。IL1β在肝癌组织中和对照组的表达强度及频度分别为±和±。3种细胞因子在肝癌组织中有5种不同表达方式。结论：原发性肝癌患者的抗肿瘤免疫存在严重缺陷，细胞因子间的平衡被打破，使已处于抑制状态的Th细胞免疫更趋恶化，致使肿瘤细胞容易发生肝内转移及术后复发。【关键词】肝肿瘤・白细胞介素1・白细胞介素4・干扰素Ⅱ型・肿瘤转移・肿瘤复发，局部Therelationshipbetweenimmunoregulatorycytokinesandintrahepaticmetastasisandrecurrenceinprimaryhepatocellularcarcinoma【ABSTRACT】Objective:Toobservetheexpressionofimmunoregulatorycytokinesincludinginterleukin4(IL4,Th2)、interferonγ(IFNγ,Th1)、interleukinbetal(IL1β)inthetissuesofhepatocellularcarcinoma(HCC),andtoinvestigatetherelationshipbetweenthechangesofimmunoregulatorycytokinesinpatientswithhepatocellularcarcinomaandintrahepaticmetastasisand:Theimmunoregulatorycytokines:IFNγ(Th1)、IL4(Th2)、IL1βwereanalyzedby:34caseswithHCCwerecomparedwiththeoneswithlivercirrhosis,theexpressionratesanddensityofIFNγ(Th1)inthetissuesofhepatocellularcarcinomaweremuchlowerthanthatinthelivercirrhosis,theyare±(18/34,%)and±(12/15,%),issignificantdifferencebetweenthem(P＜).TheexpressiondensityandlevelsofIL4(Th2)inthetissuesofhepatocellularcarcinomaincreasedclearly,comparedwiththecontrolare±and±,differenceshowedstatisticalsignificance(P＜).TheexpressionratesandlevelsofIL1βinthetissuesofhepatocellularcarcinomaandlivercirrhosiswere±(30/34,%)and±(4/15,%),wassignificantdifferencebetweenthem(P＜).Abovethreeimmunoregulatorycytokinesinpatientswithhepatocellularcarcinomashowedfivedifferentexpression:Thereisseveredeficiencyofimmuneresponsesagainsttumorsinpatientswithhepatocellularcarcinoma,thebalancebetweenthoseaboveimmunoregulatorycytokineswasdestroyed,sothatimmuneresponsesagainsttumorswereweakerandinfurthersuppression,whichmayenhancetheincidenceratesofintrahepaticmetastasisandrecurrenceofhepatocellularcarcinomaafteroperation.【KEYWORDS】Liverneoplasms・Interleukin1・Interleukin4・InterferontypeⅡ・Neoplasmmetastasis・N