预览加载中,请您耐心等待几秒...
1/10
2/10
3/10
4/10
5/10
6/10
7/10
8/10
9/10
10/10

在线预览结束,喜欢就下载吧,查找使用更方便

如果您无法下载资料,请参考说明:

1、部分资料下载需要金币,请确保您的账户上有足够的金币

2、已购买过的文档,再次下载不重复扣费

3、资料包下载后请先用软件解压,在使用对应软件打开

不同鲜食葡萄品种花粉生活力测定及贮藏特性研究摘要:[目的]花粉生活力的高低直接影响葡萄杂交育种效率的高低或成败。快速准确测定不同鲜食葡萄品种的花粉生活力,可为正确选择杂交组合和授粉时间等提供参考依据,也是保证杂交育种顺利进行的关键。[方法]以新鲜葡萄花粉为试材,用离体培养法测定花粉生活力,并探讨不同贮藏条件对鲜食葡萄花粉萌发的影响。[结果]通过镜检发现基拉尔花粉败育;不同品种的花粉生活力存在差异,自然阴干条件下供试品种花粉的生活力高低顺序为:郑州早红>芳香拉查基>瑞比尔>泽玉>京秀,其花粉生活力分别为50.41%、46.22%、43.04%、40.35%和24.13%,京秀与其它4个品种差异极显著。在不同的贮藏条件下,花粉生活力随着贮藏时间的延长而下降,不同的贮藏条件对其有很大的影响,适当的低温可有效延长花粉贮藏时间。其中-40℃萌发率最高,为最佳贮藏方法;在贮藏过程中,泽玉和京秀存在“短期被迫休眠现象”。关键字:鲜食葡萄,花粉,生活力,贮藏StoragelifeandPollenViabilityTestofDifferenttablegrapeCultivarsAuthorLiuJun-liSupervisorLiGui-rong(SchoolofHorticultureandLandscapeArchitecture,HenanInstituteofScienceandTechnology,XinxiangHenan453003)Abstract:[0bjeetive]Theefficiencyandthesuccessorfailureofhybridizationforgrapewereinfluencedbypollen’sgerminationability.Hence,themethodsformeasuregrapepollen’sgerminationabilitywhichisquicklyandaccuratecouldprovidereferencesforcrosscombinationandpollinationtime.alsoitisthekeyforsuccessofbridization.[Method]Withthefreshpollensofgrapeasthetestedmaterials,determinationofpollenviabilitybytestingmethodofpollensculturedinvitro,andtheeffectsofdifferentstorageconditiononthepollengerminationoftable-grapewerealsodiscussed.[Result]Itwasfoundthatkielarpollenabortionassayedundermicroscope.thepollenviabilityofdifferentofvarietiesaredifferent.Naturaldryingconditionstestedviabilityofpollenoftheorderfromhightolowis:zhengzhouzaohong>芳香拉查基>riber>zeyu>jingxiu,Thepollenviabilitywere50.41%,46.22%,43.04%,40.35%and24.13%.jingxiuandtheother4varietiesshowedsignificantdifference.Underdifferentstorageconditions,vitalityofpollenweregraduallydescendaslastofstoretime,differentstorageconditionshaveaimpactonits,thesuitablelowtemperatureeffectiveextensionthestoretimeofpollen.Thebestmethodstokeepvitalityofpollenat-40℃,thegerminationofpollenishigher;duringprocessofstorage,theappearanceof“compelleddormancyofshort-term”wasexistofzeyuandjingxiu.Keywords:table-grape,pollen,viability,storage1引言葡萄(Grapes),又名蒲桃、草龙珠、山葫芦、李桃,为葡萄科(VitaceaeLindley)葡萄属(VitisL.),多年生落叶HYPERLINK"http://baike.baidu.c