HPLC法测定大黄中游离蒽醌的含量.docx

HPLC法测定大黄中游离蒽醌的含量DeterminationoffreeinRhubarbbyHPLC专业：姓名：学号：指导老师：年月日目录TOC\oHYPERLINK\l"_Toc833"摘要PAGEREF_Toc833\h\*Arabic1HYPERLINK\l"_Toc4458"1仪器与试药PAGEREF_Toc4458\h\*Arabic2HYPERLINK\l"_Toc30708"2方法与结果HYPERLINK\l"_Toc16566"2.1供试品溶液的制备PAGEREF_Toc16566\h\*Arabic2HYPERLINK\l"_Toc8959"2.2对照品溶液的制备PAGEREF_Toc8959\h\*Arabic3HYPERLINK\l"_Toc29032"2.3显色剂的制备PAGEREF_Toc29032\h\*Arabic3HYPERLINK\l"_Toc25995"2.4检测波长的确定PAGEREF_Toc25995\h\*Arabic3HYPERLINK\l"_Toc28026"2.5标准曲线的绘制PAGEREF_Toc28026\h\*Arabic3HYPERLINK\l"_Toc24921"2.6总蒽醌的含量测定PAGEREF_Toc24921\h\*Arabic3HYPERLINK\l"_Toc30885"2.6.1供试品溶液的制备PAGEREF_Toc30885\h\*Arabic3HYPERLINK\l"_Toc22580"2.6.2精密度考察PAGEREF_Toc22580\h\*Arabic4HYPERLINK\l"_Toc3745"2.6.3稳定性考察精密吸取PAGEREF_Toc3745\h\*Arabic4HYPERLINK\l"_Toc23488"2.6.4重复性考察PAGEREF_Toc23488\h\*Arabic4HYPERLINK\l"_Toc5149"2.6.5总蒽醌含量测定PAGEREF_Toc5149\h\*Arabic4HYPERLINK\l"_Toc30984"2.7游离蒽醌的含量测定PAGEREF_Toc30984\h\*Arabic4HYPERLINK\l"_Toc6318"2.7.1供试品的制备PAGEREF_Toc6318\h\*Arabic4HYPERLINK\l"_Toc9530"2.7.2精密度考察取PAGEREF_Toc9530\h\*Arabic4HYPERLINK\l"_Toc30987"2.7.3稳定性考察取PAGEREF_Toc30987\h\*Arabic5HYPERLINK\l"_Toc7530"2.7.4重复性考察PAGEREF_Toc7530\h\*Arabic5HYPERLINK\l"_Toc27723"2.7.5游离蒽醌含量测定取PAGEREF_Toc27723\h\*Arabic5HYPERLINK\l"_Toc14683"3讨论PAGEREF_Toc14683\h\*Arabic5HYPERLINK\l"_Toc7176"3.1芦荟大黄素PAGEREF_Toc7176\h\*Arabic6HYPERLINK\l"_Toc29080"3.2大黄酸PAGEREF_Toc29080\h\*Arabic6HYPERLINK\l"_Toc9869"3.3大黄素PAGEREF_Toc