马铃薯脱毒种薯生产技术规程.docx

DB××/T××××—××××四川省质量技术监督局发布2008-9-1实施2008-6-6发布马铃薯脱毒种薯生产技术规程TechnicalProceduresofVirus-freeSeedPotatoProductionDB51/T818—2008DB51四川省地方标准ICS65.020.20B23DB51/T818—2008PAGEII目次前言----------------------------------------------------------------------------------------------------------------------------Ⅱ1.范围---------------------------------------------------------------------------------------------------------------------------12.规范性引用文件------------------------------------------------------------------------------------------------------------13.术语和定义------------------------------------------------------------------------------------------------------------------14.脱毒组培苗快繁------------------------------------------------------------------------------------------------------------25.原原种生产-------------------------------------------------------------------------------------------------------------------36.原种生产----------------------------------------------------------------------------------------------------------------------47.脱毒种薯繁育体系（见附录G）----------------------------------------------------------------------------------------4附录AMS培养基贮备液的配制----------------------------------------------------------------------------------------5附录B（规范性附录）酶联免疫吸附试验法------------------------------------------------------------------------6附录C（规范性附录）往复双向聚丙烯酰胺凝胶电泳法---------------------------------------------------------8附录D种薯切块操作程序----------------------------------------------------------------------------------------------12附录E主要病虫害防治--------------------------------------------------------------------------------------------------12附录F（规范性附录）马铃薯主要虫害症状与防治方法--------------------------------------------------------13附录G脱毒种薯繁育体系----------------------------------------------------------------------------------------------15前言本标准附录B、附录C、附录F为规范性附录。本标准由四川省农业厅提出并归口。本标准由四川省质量技术监督局批准。本标准由四川省农业技术推广总站