(毕业论文)蛹虫草菌丝体液体培养条件的优化正文终稿.doc

长春工业大学毕业论文34摘要蛹虫草，也称北冬虫夏草，具有补虚损、益精气、保肺、益肾、止血化痰、滋补强壮等功效，是我国的名贵中草药材。本论文采用沈阳微生物研究所提供的蛹虫草优质菌种，通过斜面培养、液体培养等过程，获得优质的蛹虫草菌丝体，并以菌丝体重量为指标，对培养温度、初始pH值、培养箱转速、最佳碳源、最佳氮源、最佳无机盐等液体培养条件进行单因素分析，通过正交试验法确立了蛹虫草菌丝体的最佳培养基配方。实验结果表明，蛹虫草菌丝体培养最佳碳源是葡萄糖，最佳氮源是蛋白胨，最佳无机盐是KH2PO4和MgSO4；最佳培养基配方是葡萄糖20g/L，蛋白胨10g/L，KH2PO41.0g/L，MgSO40.5g/L，VB150mg/L；最佳培养温度是24℃，最佳初始pH值是6.0，最佳培养箱转速时150r/min；并在最佳培养条件下绘制了蛹虫草菌丝体生长曲线，其对数期为第5～7.5天，菌丝体平均重为7.07g，稳定期为第7.5～9天，菌丝体重为14.63g，从第9天后，蛹虫草菌丝体开始衰退。关键字：蛹虫草；液体培养；正交试验法AbstractCordycepsmilitaris,alsoknownastheNorthCordyceps,hastheeffectofcompletingdeficiency,benefitingtheessence,lungandkidney,haemostaticandresolvingphlegm,nourishingandstrong,ItisapreciousChineseherbalmedicinematerials.CordycepsmilitarisinthispaperhashighqualityandisprovidedbytheShenyangInstituteofMicrobiology.Aftertakingslantcultureandliquidcultureprocesses,wegethighqualityCordycepsmilitarismycelia.Ittakesmyceliumweightasanindicatortomakeunivariateanalysisoftheincubationtemperature,initialpHvalueincubatorspeed,thebestcarbonsource,thebestnitrogensourceandthebestinorganicsaltsliquidcultureconditions.Withorthogonaltestmethod,it’sestablishedtheoptimummyceliamedium.Theexperimentalresultsshowthatthebestcarbonsourceforthemyceliaisglucose,thebestnitrogensourceispeptone,thebestinorganicsaltisKH2PO4andMgSO4.Aoptimummediumincludesglucose20g/L,peptone10g/L,KH2PO41.0g/L,MgSO40.5g/L,VB150mg/L.Theoptimalincubationtemperatureis24℃,theoptimalinitialpHvalueis6.0,thebestincubatorspeedis150r/min.ThegrowthcurveofCordycepsmilitarismyceliaisdrawnontheoptimalcultureconditions.Thelogarithmicphaseturnedupatthe5thto7.5thday,themyceliumaverageweighted7.07g,thestabilizationperiodturnedupatthe7.5thto9thday.Themyceliumweighted14.63gandtherecessionbeganfromtheninthday.Keywords:Cordycepsmilitaris;Liquidculture;Orthogonalmethod.目录摘要IAbstractII第一章前言1第二章文献综述22.1蛹虫草简介22.1.1形态特征22.1.2生长条件32.1.3功能成分32.1.4药用价值52.2蛹虫草培养基的作用72.2.1碳源72.2.2氮源72.2.3无机盐72.3蛹虫草培养条件的影响72.3.1初始PH值的影响72.3.2培养温度的影响82.3.3摇瓶转速的影响82.4菌种液体培养及优化82.4.1单因素试验法82.4.2正交试验设计法82.4.3响应面优化设计