MTT法检测滑菇培养基在不同酸碱度条件下菌丝生物量的研究 定稿.doc

摘要本次实验目的是利用MTT法来检测滑菇培养基菌丝与琥珀酸脱氢酶（SDH）活性之间的关系从而推出滑菇培养基在不同酸碱度时的菌丝生物量。实验以滑菇5188菌种为实验材料首先通过筛选找到合适的培养基配方和菌丝培养方法其次将液体培养好的滑菇菌丝球分别称取0.05g、0.1g、0.15g、0.2g、0.25g、0.3g、0.35g、0.4g利用MTT法检测出琥珀酸脱氢酶（SDH）与滑菇菌丝之间的标准曲线以石灰（0%、1%、2%、3%、4%、5%、6%）来调节固体培养基的酸碱度然后以此曲线为标准得出滑菇固体培养基菌丝在不同酸碱条件下其菌丝生物量是否有变化。实验结果表明：石灰量为1%-3%的区间菌丝生物量总体呈上升趋势在石灰量为3%时达到最高。而当石灰量达到4%及以上时菌丝生物量有所下降。所以培养基石灰含量3%最适合菌丝生长。本次实验是为MTT法测定食用菌菌丝生物量提供理论参考。关键词：滑菇；MTT；菌丝生物量；琥珀酸脱氢酶AbstractThepurposeofthisexperimentistousetheMTTmethodtodetecttherelationshipbetweenthemyceliumofthemushroommediumandtheactivityofsuccinatedehydrogenase(SDH)soastointroducethemycelialbiomassofthemushroommediumatdifferentpHlevels.Intheexperiment5188strainsofslidingmushroomswereusedasexperimentalmaterials.Firstthesuitablemediumformulationandmyceliumcultivationmethodwerefoundthroughscreening.Secondlytheliquidculturedslidingmushroommyceliumballswereweighed0.05g0.1g0.15g0.2respectively.g0.25g0.3g0.35g0.4gusingtheMTTmethodtodetectthestandardcurvebetweensuccinatedehydrogenase(SDH)andthemushroomhyphaewithlime(0%1%2%3%4%5%6%)toadjustthepHofthesolidmediumandthenusethiscurveasastandardtoobtainwhetherthemycelialbiomassofthemyceliaofthesolidmediumofthepleurotusostreatushaschangedunderdifferentacidandalkaliconditions.Theexperimentalresultsshowthat:intherangeof1%-3%oflimethemycelialbiomassgenerallyshowsanupwardtrendreachingthehighestwhentheamountoflimeis3%.Whentheamountoflimereached4%andabovethemycelialbiomassdecreased.Thereforethemediumwithalimecontentof3%ismostsuitableformycelialgrowth.ThisexperimentistoprovideatheoreticalreferencefortheMTTmethodtodeterminethebiomassofediblefungusmycelium.Keywords:Pholiotanameko；MTT；Mycelialbiomass；Succinatedehydrogenase目录1引言12材料和方法22.1材料22.1.1供试菌种22.1.2主要实验原料22.1.3主要药品试剂32.1.4仪器设备32.2方法