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(Protein)strukturanalysehttp://www.ncbi.nlm.nih.gov/entrezURLfunktioniertgerademalnichtLiteraturAccessableSizeRegimesInteractionofRadiationwithMatterInteractionofRadiationwithMatterAtomicScatteringfactors/lengthComparisonNeutron-andX-ray-scatteringlengthsomerelevantelements[10-12cm]NeutronScatteringLengthofbiologicalrelevantelements[10-12cm]NeutronsX-RaysIntensitylowhighH-sensitivityhighnoneIsotope-sensitivitystrongnoneHeavyelementslowhighSpin-sensitivitystrongaveragePenetrationdepthhighlowSamplesize/amountlargesmallMeasurementtimelongshortInteractionwithnucleielectronshellelectronshellunsystematicaZRadiationdamagenonehighToRemember:ContrastVariationTheConceptofContrastVariationX-RayScatteringTheConceptofContrastVariationExamples:Polymer-SurfactantInteractionInternalContrastVariationHExamples:Polymer-SurfactantInteractionCompositionofbacterialribosomesD.I.Svergunetal.:JMB271(1997)602-618D.I.Svergunetal.:JMB271(1997)602-618D.I.Svergunetal.:JMB271(1997)602-618NeutronScatteringLengthofbiologicalrelevantelements[10-12cm]SpinContrastVariationInsituStructuresof14ProteinsYusupovetal.:Science292(2001)883-896Break