预览加载中，请您耐心等待几秒...

Rabies virus nucleoprotein as a carrier for.pdf

预览
1/6
2/6
3/6
4/6
5/6
6/6

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

RabiesvirusnucleoproteinasacarrierforforeignantigensMartinL.Koser*JamesP.McGettigan*GeneS.Tan†MaryEllenSmith†HilaryKoprowski†BernhardDietzschold†andMatthiasJ.Schnell*‡Departmentsof*BiochemistryandMolecularPharmacologyand†MicrobiologyJeffersonMedicalCollegeThomasJeffersonUniversityPhiladelphiaPA19107-5541ContributedbyHilaryKoprowskiMay182004Rabiesvirus(RV)nucleoprotein(N)tightlyencapsidatesthePartoftheimmuneprotectionfrompathogensrequiresatgenomicandantigenomicRNAofRVtoformtheviralribonucle-leastastrongBcell-mediatedhumoralresponse.Ithasbeenoprotein(RNP)complex.AntigenssuchasNpresentedinahighlypreviouslyshownwithdifferentvirus-basedexpressionsystemsorganizedstructurearesufficientandevendesirabletoactivateBthatavarietyofviralparticlesorvirus-likeparticlescanserveascellstoproliferateandproduceantibodies.Inadditiontoactivat-carriersofforeignBcellepitopeswithintheviralcapsidoringBcellstoproliferateithasbeenshownthatRVNintheRNPenvelopeproteinoftheviralparticle.TheseepitopecarrierscomplexinducespotentThelpercellresponsesresultinginlong-resultedinstrongBcellresponsesagainsttheexpressedforeignlastingandstronghumoralimmuneresponsesagainstRV.Theepitopesindicatingausefulnessofcarrierproteins(14–20).possibilitytosystematicallyincorporateforeigngenesintotheHowevermostoftheseapproachesarerestrictedtoincorpora-genomeofRVandproducearecombinantvirusallowsustotionofonlyshortpeptidesthatdefineepitopesduetotheexaminewhethertheimmunogenicityofforeignantigenscanberestrictedcloningcapacitywithinthecarrierproteins.Wehy-enhancedbyincorporationintotheRVRNPstructure.TotestthispothesizedthatRVNasacarrierproteinforforeignepitopeshypothesisweconstructedarecombinantRVexpressingaRVmightcircumventthisproble