血管内皮生长因子和雌二醇促进血管内皮祖细胞分化生成血管的对比研究.docx

血管内皮生长因子和雌二醇促进血管内皮祖细胞分化生成血管的对比研究[摘要]目的：对比研究常规剂量下血管内皮生长因子（VEGF）和雌二醇对血管内皮祖细胞（EPCs）分化生成血管的促进作用。方法：分离培养人外周血EPCs与基质胶混匀后注射到9只裸鼠双侧下腹部另设2只注射等体积培养液与基质胶的混合液。将9只注射细胞的裸鼠随机分为3组每组分别定期局部注射VEGF、雌二醇生理盐水定期观察记录血管组织块的生长状况。移植6周后取材测量计算血管组织块的体积、HE染色观察血管组织块的血管增殖状况各组之间进行对比。结果：给药组血管组织块体积与注射生理盐水组相比具有显著性差异体积明显偏大而给药组之间体积未见显著性差异。HE染色观察可见各组的血管组织块内血管增殖明显血管排列紊乱的管腔大小不一。给药组血管密度明显大于注射生理盐水组而给药组之间的血管密度差异较小。结论：VEGF和雌二醇组具有促进EPCs分裂增殖形成血管的能力常规剂量下两者促进EPCs分裂增殖生成血管的能力无显著性差异。[关键词]内皮祖细胞；血管；血管内皮生长因子；雌二醇。[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2013）01-0048-03ComparativestudyofvascularendothelialgrowthfactorandestradiolinpromotingendothelialprogenitorcellsdifferentiationandgenerationbloodvesselsDONGYongLIWen-zhiXINYiSUNZhi（DepartmentofPlasticSurgeryandLaserMedicineBeijingAnzhenHospitalofCapitalMedicalUniversityBeijingInstituteofCardiopulmonaryandVascularDiseaseBeijing100029China）Abstract：ObjectiveTocomparetheabilityofvascularendothelialgrowthfactor（VEGF）andestradiolinpromotingendothelialprogenitorcellsdifferentiationandgenerationbloodvesselsundercommondoses.MethodsToisolateandculturehumanperipheralbloodEPCsfirstthentomixewiththematrigelandtransplantetothelowerabdomensofninenudemicetodividetheninenudemiceintothreegroupsaccordingtoarandomgroupingtoinjectVEGFestradiolandsalineataregulartimetoobserveandrecordthegrowingstatusofvasculartissueregularlytodrawthevasculartissueaftersixweekstoobservetheorganizationalstructurebyHEstainingthencontrastwiththegroups.ResultsThevasculartissuevolumegroupsinjectedofdrugshavesignificantdifferencewiththegroupsinjectedofsalinetheyhavebiggervolumetocontrastthegroupsinjectedofsalinebuthevasculartissuevolumebetweenthegroupsinjectedofdrugsisnosignificantdifference.BydrawningHEstainingthevasculartissueshaveproliferationalmussybloodvesselsThevascular