肺宁对大鼠博莱霉素肺损伤早期治疗作用的机制研究.docx

肺宁对大鼠博莱霉素肺损伤早期治疗作用的机制研究作者：梁向艳田琼李安李超锋杜晓晗【关键词】肺宁；博莱霉素；肺泡炎；肺纤维化【Abstract】AIM:TostudytheeffectofChinesemedicineFeiningonearlyexperimentalpulmonaryfibrosisinratsinducedbybleomycin(BL).METHODS:AllratswererandomlydividedintocontrolgroupmodelgroupFeininggroups(dose:124g/kg)prednisonegroupandgivenBLendotracheallyexceptthenormalcontrolgroup.Onday2eachBLgroupwasgivenFeiningandprednisonerespectively.Onday7and146ratsineachgroupwerekilledandlungswereharvested.Therightlungsweresubjectedtohistopathologicalstudies(HEstaining)theleftlungswerehomogenizedforbiochemicalassaysofSODactivityandMDAGSHTNFαcontents.RESULTS:ComparedtomodelgroupalveolitiswasalleviatedineachFeininggroupsignificantly;onday7MDATNFαcontentsofhomogenizedlungsineachFeininggroupdecreased(P<0.05)GSHcontentofhomogenizedlungsintheFeining(4g/kg)groupandprednisonegroupincreased(P<0.05);onday14SODactivityGSHcontentofhomogenizedlungsineachtreatedgroupincreased(P<0.05)MDAcontentsdecreased(P<0.05)TNFαcontentofhomogenizedlungsintheFeining(24g/kg)groupdecreased(P<0.05).CONCLUSION:ThemechanismbywhichFeiningresistspulmonaryalveolitisisinvolvedinpromotingtheactivityofSODreducinglipidperoxide（LPO）mediatedinjuryenhancingthepowerofeliminatingoxygenradicalimprovingtheoxidation/deoxidationbalance.Feiningcanpreventpulmonaryfibrosis.【Keywords】feining;bleomycin;alveolitis;pulmonaryfibrosis【摘要】目的：观察中药肺宁对博莱霉素诱导的大鼠肺纤维化早期的防治作用.方法：以博莱霉素气管内注入建立肺纤维化模型次日始分别给与中药肺宁和强的松灌胃治疗于给药第714日处死动物右肺组织行病理组织学检查左肺组织匀浆进行超氧化物歧化酶（SOD）活力、丙二醛（MDA）、谷胱甘肽（GSH）、肿瘤坏死因子（TNFα）含量检测.结果：与博莱霉素对照组相比肺宁不同剂量组肺泡炎的程度均明显减轻；7d时肺宁各组肺组织匀浆MDATNFα含量降低（P<0.05）肺宁高剂量组(4g/kg)、强的松组肺组织匀浆GSH含量升高（P<0.05）；14d时肺宁各剂量组、强的松组肺组织匀浆SOD活力、GSH含量均增高（P<0.05）MDA含量降低（P<0.05）肺宁中剂量组(2g/kg)、高剂量组(4g/kg)TNFα含量降低（P<0.05）.结论：肺宁抗肺纤维化的作用机制与提高SOD的活性降低脂质过氧化物（LPO）损伤有关通过提高