羊膜自噬的形态及其相关基因表达.docx

羊膜自噬的形态及其相关基因表达[摘要]目的：阐明羊膜上皮细胞自噬过程的形态及其相关基因表达。方法：20片足月羊膜用透射电镜检查用单丹磺酰戊二胺（MDC）标记自噬泡和溶酶示棕红（LTR）检查溶酶体；用RT-PCR检测beclin-1apg-5和DAPK基因的转录。结果：在羊膜上皮可见多数双层膜包被的自噬泡它可分为两种：一种是自噬体其中包含细胞浆成份；另一种是自噬溶酶体系自噬体和溶酶体融合细胞浆成份降解。MDC和LTR染色阳性。beclin-1apg-5和DAPK基因在多数羊膜上皮有表达。结论：在正常羊膜上皮出现自噬并有相关基因表达羊膜上皮为适应其营养限制可诱导自噬以促进自我营养。[关键词]羊膜；自噬；自噬体；自噬溶酶体[中图分类号]R714.56[文献标识码]A[文章编号]1673-7210（2008）08（a）-011-03MorphologyofautophagyandexpressionofsomerelativegenesinamnionLUShan-qun1SHENZhong-ying2（1.DepartmentofObstetricsCentralHospitalofShantouShantou515011China;2.DepartmentofPathologyMedicalCollegeofShantouUniversityShantou515011China）[Abstract]Objective:Toexposethemorphologyofautophagyandexpressionofsomeassociatedgenesintheamnion.Methods:Twentypiecesofamnionsintermgestationwereexaminedbytransmittedelectronmicroscopyandbymono-dansylcadaverin(MDC)andLysoTrackerRed(LTR)stainingmarkersforautophagicvacuoleandlysosome.Autophagy-associatedgenesbeclin-1apg-5andDAPKwereassayedbyRT-PCR.Results:Mostautophagicvacuolesenclosedwithdoublemembraneswerefoundintheamnioticepithelialcells.Theyweredividedtwokinds:autophagosomesinwhichcontainedsomecytoplasmiccompartmentsandautolysosomesinwhichautophagosomefusedwithlysosomesandcontaineddegradedcytoplasmiccompartments.MDCandLTRstainingwaspositiveinthecytoplasm.beclin-1apg-5andDAPKgeneswereexpressedingreatproportionofamnioticepithelium.Conclusion:Theseresultsdisplaythatthephenotypeofautophagyareinducedinnormalamnioticepitheliumwithsomeautophagy-associatedgenesexpressionandsuggestthatamnioticepitheliaadapttothenutrientlimitationbyinducingautophagytopromoteself-nourishment.[Keywords]Amnion;Autophagy;Autophagosome;Antolysosome羊膜是子宫内包被胎儿的一层薄膜对胎儿有保护作用。羊膜是一种生物膜它结构简单仅有一层上皮细胞位于基膜上及其下的一薄层结缔组织由于羊膜无血管供应细胞通过羊水营养物质维生故其有耐“饥饿”的特点。由此羊膜可用于临床多种用途：如覆盖创面用在烧伤和眼