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益气活血方药对老年大鼠肝脏衰老相关基因表达的影响.docx

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益气活血方药对老年大鼠肝脏衰老相关基因表达的影响作者：黎志萍赵伟康徐品初陈方敏金国琴姚明忠【关键词】肝细胞［摘要］目的：观察益气、活血方药对老年大鼠肝脏衰老相关基因表达的影响。方法：以自然衰老大鼠为衰老模型采用RTPCR、Westernblotting法研究益气、活血方药对老年大鼠肝脏p16、p21、增殖细胞核抗原（proliferatingcellnuclearantigenPCNA）、cyclinD1和cyclinEmRNA转录和蛋白表达的影响。结果：老年大鼠肝脏p16、p21、cyclinD1mRNA转录和蛋白的表达增加；PCNA、cyclinEmRNA转录和蛋白的表达降低。与老年大鼠比较益气、活血方药可下调老年大鼠肝脏cyclinD1mRNA转录和蛋白表达上调促进增殖基因cyclinEmRNA转录和蛋白表达但对p16、p21和PCNA的调节作用不明显。结论：益气、活血方药主要通过调控cyclinD1和cyclinEmRNA转录和蛋白表达而促进细胞增殖。［关键词］衰老大鼠;肝细胞;p16;p21;增殖细胞核抗原;cyclinD1;cyclinE;益气方;活血方EffectsofrecipesforreplenishingqiandactivatingbloodonsenescencerelatedgeneexpressionsintheliverofagingratsABSTRACTObjective:Toobservetheeffectsofrecipesforreplenishingqiandactivatingbloodonp16p21proliferatingcellnuclearantigen(PCNA)cyclinD1andcyclinEgeneexpressionsintheliverofagingrats.Methods:Arecipeforreplenishingqiandarecipeforactivatingbloodwereadministeredtoagingratsrespectivelyandtheeffectsoftheaboverecipesontheexpressionsofsenescencerelatedgenes(p16p21PCNAcyclinD1andcyclinE)wereexaminedbyRTPCRandWesternblottingmethods.Results:Theexpressionsofp16p21andcyclinD1mRNAsandproteinsintheliveroftheuntreatedagingratswereupregulatedwhiletheexpressionsofPCNAandcyclinEmRNAsandproteinsdecreased.AscomparedwiththeuntreatedagingratsbothrecipescoulddownregulatetheexpressionsofcyclinD1mRNAandproteinandupregulatetheexpressionsofcyclinEmRNAandproteinbuthadnoobviouseffectsontheexpressionsofmRNAsandproteinsofp16p21andPCNA.Conclusion:RecipesforreplenishingqiandactivatingbloodcanimprovethelivercellproliferationofagingratsviadownregulatingtheexpressionsofcyclinD1mRNAandproteinandupregulatingtheexpressionsofcyclinEmRNAandprotein.KEYWORDSagingrats;hepatocytes;p16;p21;proliferatingcellnuclearantigen;cyclinD1;