灯盏生脉胶囊对脑缺血再灌注损伤大鼠的保护作用.docx

灯盏生脉胶囊对脑缺血再灌注损伤大鼠的保护作用[摘要]目的：研究灯盏生脉胶囊对脑缺血再灌注损伤的保护作用。方法：将大鼠随机分为假手术组、模型组和灯盏生脉胶囊组。采用改良的ZeaLonga线栓法制作大鼠大脑中动脉阻塞模型缺血后24h灌胃给予灯盏生脉胶囊于缺血后7、14、21d分别测定神经功能缺损评分和体重增长率于缺血后21d采用TTC法测定脑梗死体积百分比免疫组织化学法检测脑组织血管内皮生长因子（VEGF）的表达。结果：与模型组比较灯盏生脉胶囊组神经功能缺损评分、体重增长率、脑梗死体积百分比及VEGF的表达差异均有统计学意义（均P＜0.05）。结论：灯盏生脉胶囊对大鼠脑缺血再灌注损伤有明显的保护作用可能与其促进VEGF表达有关。[关键词]灯盏生脉胶囊；脑缺血再灌注损伤；血管内皮生长因子[中图分类号]R968[文献标识码]A[文章编号]1673-7210（2011）03（c）-055-03ProtectiveeffectsofDengzhanShengmaiCapsulesonfocalcerebralischemiareperfusioninjuryofratsHUANGYizhi1CHENChao2HUANGChunhui1CHENDandan11.LaboratoryCentreofGuangxiHealthCollegeNanning530023China;2.DepartmentofPharmacyFirstClinicAffiliatedHospitaltoGuangxiTraditionalChineseMedicineNanning530023China[Abstract]Objective:ToinvestigatetheprotectionofDengzhanShengmaiCapsulesonischemiareperfusioninjuryofrats.Methods:Ratsweredividedinto3groupsinrandom:shamoperationgroupmodelgroupDengzhanShengmaiCapsulesgroup.Middlecerebralarteryocclusion(MCAO)modelwasestablishedaccordingtoZeaLonga＇spattern.RatswerefedwithDengzhanShengmaibyintragastricadministrationafter24hofischemia.Thebodygrowthrateandneuroethologyfunctionofratsweretestedon7d14dand21d.CerebralinfarctvolumewasdeterminedusingTTCcoloration.Immunohist-ochemicalmethodwasusedtodetectedtheexpressionofVEGF.Results:TheneuroethologyfunctioninfarctvolumeandtheexpressionofVEGFofratswereobviouslydifferentbetweenDengzhanShengmaiCapsulestreatedgroupandthemodelgroup(allP＜0.05).Conclusion:DengzhanShengmaiCapsulesplaysaprotectiveeffectsonfocalcerebralischemiareperfusioninjuryofratswhichmaybecorrelatedtotheup-regulationofexpressionofVEGF.[Keywords]DengzhanShengmaiCapsules;Cerebralischemiareperfusioninjury;Vascularendothelialgrowth[作者简介]黄一挚（1982-）女广西南宁助理讲师药学专业主要从事药物制剂教学工作。脑缺血损伤基本病机是由于急性血栓形成