晚期肿瘤患者淋巴细胞亚群特点及临床意义.docx

晚期肿瘤患者淋巴细胞亚群特点及临床意义[摘要]目的：通过观察晚期肿瘤患者外周血淋巴细胞亚群特点探讨其免疫功能状况及临床意义。方法：应用流式细胞仪检测56例晚期肿瘤患者（肿瘤组）及42例健康体检者（健康组）外周血淋巴细胞亚群计数并对其作比较。结果：肿瘤组较健康组T细胞亚群CD8+比值上升CD4+、CD4+/CD8+比值下降差值有统计学意义（P＜0.05）CD3+比值升高差异无统计学意义（P＞0.05）B细胞亚群下降差异有统计学意义（P＜0.05）NK比值下降差异无统计学意义（P＞0.05）。结论：晚期肿瘤患者的细胞免疫及体液免疫功能均低于健康人其中体液免疫下降较明显对临床应用提高免疫力药物也具有指导意义。[关键词]淋巴细胞亚群；免疫功能；流式细胞术[中图分类号]R730.43[文献标识码]B[文章编号]1673-7210（2011）11（c）-094-02ClinicalcharacteristicsandsignificanceoflymphocytesubsetsinpatientswithadvancedcancerZHENGShujun1JIAXihua2WANGYunliang2GUOJianmei21.ChengdeMedicalCollegeHebeiProvinceChengde067000China;2.DepartmentofMedicalOncologytheFirstCentralHospitalofBaodingCityHebeiProvinceBaoding071000China[Abstract]Objective:Toobservetheclinicalcharacteristicsoflymphocytesubsetsintheperipheralbloodofpatientswithadvancedcancerinordertoevaluateimmunefunctionandclinicalsignificanceoflymphocytesubsets.Methods:Lymphocytesubsetscountedinperipheralbloodof56patientswithadvancedcancerand42casesofhealthypeoplewerecomparedbytheflowcytometry.Results:CD8+ratioofpatientswithadvancedcancerwassignificantlyhigherthanthatofthehealthypeopleCD4+CD4+/CD8+ratioandBcellsubgroupweresignificantlylowerincancerpatientsthanthatofthehealthypeople(allP0.05).Conclusion:Cellularimmunefunctionandhumoralimmunefunctionofpatientswithadvancedcancerarelowerthanthatofhealthypeopleandthehumoralimmunefunctiondroppedsignificantlythisfindingisinstructiveonhowtoimprovetheclinicalapplicationofimmunitydrugs.[Keywords]Lymphocytesubsets;Immunefunction;Flowcytometry近年来肿瘤发病率逐年增加研究表明恶性肿瘤患者普遍存在免疫功能低下[1]其发生是多因素、多阶段的与机体免疫系统间的相互作用机制十分复杂[2]本文运用流式细胞术的方法检测并分析恶性肿瘤患者和健康人淋巴细胞亚群的特点探讨肿瘤对患者免疫功能的影响对临床具有指导作用。1资料与方法1.1一般资料选取2009年1月～2010年12月我院健康体检者和肿瘤患者总共98例。其中健康体检者（健康组）42例男24例女18例；年龄45~64岁平均53.2岁。肿瘤患者（肿瘤组）56例均经过病理或细胞学确诊且临床分期为Ⅳ