兔骨髓间充质干细胞定向诱导移植修复关节软骨的实验研究.docx

兔骨髓间充质干细胞定向诱导移植修复关节软骨的实验研究[摘要]目的：通过诱导兔骨髓间充质干细胞(MSCs)观察干细胞向软骨细胞的分化效果。方法：纯化兔骨髓间充质干细胞；用含转化生长因子β1的诱导液培养骨髓间充质干细胞；以MTT测定诱导细胞的增殖活性；免疫组化检测Ⅱ型胶原蛋白的表达；诱导后的细胞移植于白兔膝关节内X线摄片观察软骨修复形成情况。结果：诱导液可有效诱导MSCs向软骨细胞表型转化且骨髓间充质干细胞向软骨细胞分化所需的特异性细胞外基质――Ⅱ型胶原明显升高移植后效果明显。结论：兔骨髓间充质干细胞定向诱导后移植修复关节软骨是有效的。因此骨髓间充质干细胞有可能成为软骨组织工程较理想的种子细胞来源。[关键词]软骨细胞；骨髓间充质干细胞；移植；转化生长因子β1[中图分类号]R392.33[文献标识码]A[文章编号]1673-7210（2008）08（b）-016-03StudyonrabbitbonemarrowmesenchymalstemcellsdifferentiationtothechondrogenictotransplantforrepairingarticularcartilageLIANGXiao-pengTIANliLINaWANGZhuoTIANXiao-yeFANNi-na(ShenyangMedicalCollegeShenyang110034China)[Abstract]Objective:Toobservethedifferentiationeffectofstemcellsintochondrocytesbyinducedrabbitbonemarrowmesenchymalstemcells(MSCs).Methods:Rabbitbonemarrowmesenchymalstemcellswerepurifiedandthenculturedininducingmediumcontainingtransforminggrowthfactor-β1;MTTassaywasemployedtoevaluatetheproliferativeactivityofinducedcells;immunohistochemicalassaywasusedtodetecttheexpressionoftypeⅡcollagen;inducedcellsweretransplantedintothekneeofrabbitandX-rayphotographywasemployedtoobservetherepairandformationofcartilage.Results:MSCscouldbeeffectivelytransformedintothechondrocytesbyinducingmediumtypeⅡcollagenwhichwasaspecificextracellularmatrixandnecessaryinthedifferentiationofMSCsintochondrocytessignificantlyincreasedandtheeffectsaftertransplantationwereobvious.Conclusion:ItiseffectivethatMSCsareinducedtorepairarticularcartilageafterthetransplantation.InsummaryMSCisapossibleidealsourceofseedcellsincartilagetissueengineering.[Keywords]Chondrocytes;Mesenchymalstemcells;Transplantation;Transforminggrowthfactor-β1老年性骨关节炎、关节创伤等急、慢性疾病中常因关节损伤导致关节软骨缺损。而各种原因造成的关节软骨缺损因自身缺乏有效的修复能力损伤后常引起疼痛和关节功能障碍等。因此关节软骨损伤的修复一直是当今国内外学者极为关注的问题。骨髓间充质干细胞(mesenchymalstemcell