益气活血解毒方治疗晚期上皮性卵巢癌疗效相关靶基因筛选.pdf

益气活血解毒方治疗晚期上皮性卵巢癌疗效相关靶基因筛选李娟翁洁琼卢雯平摘要：目的采用mRNA芯片探究益氣活血解毒方维持治疗晚期上皮性卵巢癌（AEOC）气虚血瘀证疗效相关基因靶点明确其有效作用机制。方法观察AEOC气虚血瘀证患者服用益气活血解毒方维持治疗后的无进展生存期（PFS）选取PFS最长的8例为敏感组PFS最短的8例为不敏感组。采用mRNA芯片检测2组患者治疗后血液mRNA表达情况并对差异表达基因进行GO、KEGG、singal-net、path-net生物信息学分析实时荧光定量PCR（qRT-PCR）验证。结果益气活血解毒方疗效相关差异表达基因共1157个（P<0.05）其中上调基因497个下调基因660个。qRT-PCR显示MAPK3、HLA-DOA、CD40、KIR2DL3差异表达有统计学意义（P<0.05）。结论益气活血解毒方治疗AEOC气虚血瘀证患者的疗效机制可能在于抑制MAPK3及与免疫通路相关的HLA-DOA、CD40、KIR2DL3靶基因。通过对益气活血解毒方作用靶基因的探究可为AEOC气虚血瘀证微观辨证及中医精准治疗提供依据。关键词：晚期上皮性卵巢癌;mRNA芯片;益气活血解毒方;气虚血瘀证;靶基因中图分类号：R273.731文献标识码：A文章编号：1005-5304（2020）04-0028-07DOI：10.3969/j.issn.1005-5304.201910465ScreeningofTargetGenesRelatedtoEfficacyofYiqiHuoxueJieduDecoctionforAdvancedEpithelialOvarianCancerLIJuanWENGJieqiongLUWenpingGuanganmenHospitalChinaAcademyofChineseMedicalSciencesBeijing100053ChinaAbstract：ObjectiveToexplorethegenetargetsrelatedtoefficacyofYiqiHuoxueJieduDecoctionformaintenancetreatmentofadvancedepithelialovariancancer（AEOC）withqideficiencyandbloodstasissyndromeusingmRNAchip;Toclarifyeffectivemechanism.MethodsProgression-freesurvival（PFS）oftheAEOCpatientswithqideficiencyandbloodstasissyndromeaftertakingYiqiHuoxueJieduDecoctionformaintenancetreatmentwasobservedand8caseswiththelongestPFS（sensitivegroup）and8caseswiththeshortestPFS（insensitivegroup）wereselected.ApplicationofmRNAchipwasusedtodetectiontheexpressionofmRNAinpatientsbloodoftwogroups.AnddifferentiallyexpressedgeneswereanalyzedbygeneontologyKEGGsingal-netpath-netbioinformaticsanalysisandreal-timefluorescencequantitativePCR（q