养肝利胆颗粒对小鼠四氯化碳肝损伤的保护作用.docx

养肝利胆颗粒对小鼠四氯化碳肝损伤的保护作用作者：尤圣富郑培永季光魏华凤赵婕朱培庭【关键词】养肝利胆颗粒［摘要］目的：观察养肝利胆颗粒对小鼠四氯化碳（carbontetrachlorideCCl4）肝损伤的影响并探讨其作用机制。方法：采用腹腔注射CCl4制作小鼠慢性肝损伤模型。造模3周后分别予以养肝利胆颗粒小、中、大剂量胆宁片及联苯双酯治疗4周检测各组血清丙氨酸氨基转移酶（alanineaminotransferaseALT）天门冬氨酸氨基转移酶（aspartateaminotransferaseAST）肝组织超氧化物歧化酶（superoxidedismutaseSOD）活性和丙二醛（malondialdehydeMDA）含量。结果：养肝利胆颗粒可显著降低小鼠CCl4肝损伤模型血清ALT、AST的水平和肝组织MDA的含量明显升高肝组织SOD的水平。结论：养肝利胆颗粒对CCl4所致小鼠肝损伤有明显的改善作用其作用机制可能与减轻肝内脂质过氧化反应有关。［关键词］养肝利胆颗粒;四氯化碳;超氧化物歧化酶;丙二醛ProtectiveeffectsofYangganLidanGranulesoncarbontetrachlorideinducedliverdamageinmiceABSTRACTObjective:ToobservetheprotectiveeffectsofYangganLidanGranules(YGLDG)oncarbontetrachloride(CCl4)inducedliverdamageinmiceandtofindoutitsmechanism.Methods:AmodelofchronicliverdamagewasestablishedinmicebyintraperitonealinjectionofCCl4.AfterthreeweeksthosemodelmiceweretreatedwithlowmediumhighdoseYGLDGDanningTabletsandbifendaterespectivelyforfourweeks.Thenthelevelsofserumalanineaminotransferase(ALT)andaspartateaminotransferase(AST)aswellasthecontentsofsuperoxidedismutase(SOD)andmalondialdehyde(MDA)inlivertissuesweredetected.Results:YGLDGcouldsignificantlyreducethelevelsofserumALTandASTinmodelmiceandthecontentofMDAwasobviouslydecreasedwhilethecontentofSODwasincreasedinlivertissue.Conclusion:ThetherapeuticeffectofYGLDGonmicewithCCl4inducedliverdamageistorelievetheseriousnessofliverdamageanditsmechanismmayrelatetoreducingperoxidationactivityinlivertissue.KEYWORDSYangganLidanGranules;carbontetrachloride;superoxidedismutase;malondialdehyde目前具有保肝、抗肝损伤作用的中药有效成分或复方如甘草甜素、联苯双酯、当飞利肝宁和黄芪注射液等虽在临床上被广泛应用但经国际认可的该类药物均非源自我国［1］。养肝利胆颗粒（YangganLidanGranulesYGLDG）具有养肝柔肝、利胆止痛的功效临床上治疗慢性胆道感染（慢性胆囊炎、慢性胆管炎）证属肝阴不足者有一定的疗效。我们观察了养肝利胆颗粒对小鼠四氯化碳（carbontetrachlorideCCl4）慢性肝损伤的保护作用以期为临床用药提供依据并对其作用机制作进一步的探讨。1材料与方法1.1