授粉方法对克服百合_cordelia_毛百合远缘杂交障碍的影响.pdf

核农学报()～
282008,221:2831
JournalofNuclearAgriculturalSciences

文章编号:100028551(2008)012028204

授粉方法对克服百合‘cordelia’×毛百合
远缘杂交障碍的影响

马莉1张克中2张启翔1贾月慧2王哲2
(11北京林业大学,北京100083;21北京农学院,北京102206)

摘要:研究了辐照花粉辅助授粉、杂交液辅助授粉及切割花柱授粉等措施对克服百合‘cordelia’(♀)×
毛百合远缘杂交障碍的影响。结果表明:采用普通柱头授粉能够获得许多膨大果实和饱满种子,表明该
杂交组合为亲合性组合。用经1000Gy或2500Gy剂量60Co辐照的母本花粉进行辅助授粉明显地提高了
座果率和饱满种子结籽数,表明两种剂量的辐照花粉辅助授粉均能有效地克服该组合的杂交障碍。结
籽数的提高有利于后续的选育工作。采用杂交液辅助授粉,座果率和饱满种子结籽数有所提高。而切
割花柱授粉明显地减少了座果率和结籽数,表明该方法不适合于克服‘cordelia’(♀)×毛百合杂交障碍。
关键词:百合;远缘杂交;辐照花粉;切割花柱授粉;杂交液辅助授粉

THEEFFECTSOFDIFFERENTPOLLINATIONMETHODSONOVERCOMINGDISTANT
CROSSBARRIERSOFTHELILY‘CORDELIA’×Liliumdauricum

MALi1ZHANGKe2zhong2ZHANGQi2xiang1JIAYue2hui2WANGzhe2
(11BeijingForestryUniversity,Beijing100083;21BeijingAgriculturalCollege,Beijing102206)

Abstract:Theapplicationofirradiatedpollenassistingpollination,crosschemicalassistingpollinationandcut2stylepollination
inovercomingthecrossbarriersoflily‘cordelia’(♀)×Liliumdauricumwereinvestigated.Anumberoffruitsandplump
seedswereproducedbyusingthenormalstigmapollination,whichindicatedthatthecrosswasacompatiblecross.Thefact
thattheapplicationof1000Gyor2000Gyirradiatedpollenassistingpollinationincreasedthefruitsetandseedsetobviously
exhibitedthatthismethodscouldovercomethecrossbarrierseffectively.Morehybridseedswouldhaveapositiveeffectonthe
futurebreedingselection.Applicationofcrosschemical′sassistingpollinationraisedthefruitsetandseedsettosomeextent.
Thecut2stylepollinationmethoddeducedthefruitsetandseedset,whichsuggestedthatthemethodwasnotsuitablefor
bypassingthecrossbarriers.
Keywords:Lily;distantcross;irradiatedpollenassistingpollination;cut2stylepollination;crosschemical’sassisting
pollination

百合‘cordelia’是目前市场上较为流行的鲜花商业Fusariumoxyporum强[1]。用‘cordelia’与毛百合杂交,
品种,花黄色,但是抗病性差,种球贮藏过程中和连作有可能产生花色新颖、斑点增多及抗病性增强的百合
栽培时极易遭受尖孢镰刀菌(Fusariumoxyporum)侵染,新品种。该杂交组合为栽培品种与野生百合之间的杂
导致种球腐烂,影响种球贮藏和鲜切花生产。毛百合交,属远缘杂交。克服百合远缘杂交障碍的方法很多,
Liliumdauricum为原产我国东北的一种野生百合,花常用的有切割花柱授粉[2]、加热处理[3,4]、激素处理[3]、
冠钟形,为橙红色或红色,具有紫红色斑点,抗辐照花粉辅助授粉[4]、离体技术辅助杂交(如离