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响应面法优化桦褐孔菌多糖提取工艺.docx

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响应面法优化桦褐孔菌多糖提取工艺 摘要 本文研究了响应面法优化提取桦褐孔菌多糖的工艺。通过正交实验筛选出影响桦褐孔菌多糖提取率的最优因素组合，利用响应面法建立了提取率预测模型。结果表明，在最优的提取工艺条件下，桦褐孔菌多糖的提取率为8.27%。从而为桦褐孔菌多糖的高效提取及应用提供了一定的理论依据。 关键词：响应面法；桦褐孔菌多糖；正交实验；工艺优化；提取率 Abstract ThispaperstudiedtheoptimizationoftheextractionprocessofInonotusobliquuspolysaccharidesbyresponsesurfacemethodology.TheoptimalcombinationoffactorsaffectingtheextractionrateofpolysaccharidesfromInonotusobliquuswasscreenedbyorthogonalexperiment,andtheextractionratepredictionmodelwasestablishedbyresponsesurfacemethodology.TheresultsshowedthattheextractionrateofInonotusobliquuspolysaccharideswas8.27%undertheoptimalextractionconditions.ThisstudyprovidesacertaintheoreticalbasisfortheefficientextractionandapplicationofInonotusobliquuspolysaccharides. Keywords:responsesurfacemethodology;Inonotusobliquuspolysaccharides;orthogonalexperiment;processoptimization;extractionrate 1.Introduction Inonotusobliquus,alsoknownasChagamushroom,isatypeofmedicinalandediblefungusthatgrowsonthetrunkofbirchorothertreesincoldregionsoftheworld.Ithasbeenusedasatraditionalmedicineforcenturies,andhasbeenclaimedtopossessvarioushealthbenefits,suchasanti-tumor,anti-inflammatory,anti-viral,andanti-oxidativeproperties[1,2]. PolysaccharidesarethemajorbioactivecomponentsinInonotusobliquus,andhavebeenfoundtoberesponsibleformanyofitspharmacologicalactivities[3,4].Therefore,theefficientextractionofpolysaccharidesfromInonotusobliquusisofgreatimportanceforitsmedicinalandcommercialapplications. Inrecentyears,manyresearchershavefocusedontheoptimizationoftheextractionprocessofpolysaccharidesfromInonotusobliquus.Variousmethods,suchaswaterextraction,acidextraction,andenzymaticextraction,havebeenreported[5,6,7].However,theyieldandqualityoftheextractedpolysaccharidesvarygreatlydependingontheextractionconditions. Thetraditionalone-factor-at-a-timemethodiswidelyusedintheoptimizationofextractionprocesses.However,itistimeconsumingandinefficient,andcannotcapturetheinteractionsbetweendifferentfactors.Responsesurfacemethodology(RSM),which