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玉米花序发育相关基因TD1与FEA2的序列变异及其与产量相关性状的关联分析.docx

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玉米花序发育相关基因TD1与FEA2的序列变异及其与产量相关性状的关联分析 Title:SequenceVariationsofTD1andFEA2GenesinMaizeTasselDevelopmentandAssociationAnalysiswithYield-RelatedTraits Abstract: Maize(Zeamays)isoneofthemostimportantcerealcropsworldwide.Understandingthegeneticfactorsregulatingtasseldevelopmentandtheirassociationwithyield-relatedtraitsiscrucialforimprovingmaizeproductivity.Inthisstudy,weinvestigatedthesequencevariationsoftwoimportantgenes,TD1andFEA2,involvedinmaizetasseldevelopmentandexploredtheirrelationshipwithyield-relatedtraits.Throughsequenceanalysis,weidentifiedseveralvariantsinTD1andFEA2genesandexaminedtheirpotentialimplicationsonmaizeyield.Ourfindingsprovidevaluableinsightsintothegeneticbasisofmaizetasseldevelopmentandofferpotentialtargetsforfuturebreedingprogramsaimedatenhancingmaizeyield. Introduction: Maizeisastaplecropthatplaysavitalroleinensuringfoodsecurityglobally.Thedevelopmentofthetassel,themalereproductiveorganofmaize,iscloselylinkedtotheproductivityandkernelqualityofthecrop.Theregulationoftasseldevelopmentisacomplexprocessinfluencedbyvariousgeneticfactors.TheTD1andFEA2geneshavebeenidentifiedaskeyregulatorsoftasseldevelopmentinmaize.TD1,alsoknownasTeosintebranched1,controlsthebranchingpatternofthetassel,whereasFEA2,orfilamentelongation1,isinvolvedintheelongationofthetasselfilaments.Understandingthesequencevariationsinthesegenesandtheirassociationwithyield-relatedtraitsiscriticalforimprovingmaizeyieldandquality. Methods: ToinvestigatethesequencevariationsinTD1andFEA2genes,weobtainedDNAsamplesfromadiversepanelofmaizelines.PCRamplificationfollowedbyDNAsequencingwascarriedouttoidentifythevariantswithinthecodingandregulatoryregionsofTD1andFEA2.Theobtainedsequenceswereanalyzedusingvariousbioinformaticstoolstopredictthepotentialfunctionalimpactoftheobservedvariants.Additionally,wecollectedphenotypicdatarelatedtoyieldtraitsofthemaizelinesinourstudy,includingearlength,kernelnumberperear,andgrainweightperear.Statisticalanalyseswereperformedtoexaminethecorrelationsbetweentheident