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嗜水气单胞菌DBHS101基因组分析及Cpx缺失株构建和生物学特性研究.docx

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嗜水气单胞菌DBHS101基因组分析及Cpx缺失株构建和生物学特性研究 Title:GenomicAnalysisofSphingomonasaquaticastrainDBHS101andConstructionofCpx-deficientMutantsforInvestigationofBiologicalCharacteristics Abstract: SphingomonasaquaticastrainDBHS101isawaterbornebacteriumisolatedfromaquaticenvironments.Inthisstudy,weperformedgenomicanalysisofDBHS101andconstructedCpx-deficientmutantstoinvestigatetheroleoftheCpxsysteminthebacterium'sbiologicalcharacteristics.ThroughtheanalysisoftheDBHS101genome,weidentifiedseveralkeygenesandgeneticfeaturesrelatedtoitswater-adaptationandpotentialenvironmentalremediationcapabilities.Furthermore,theconstructionofCpx-deficientmutantsprovidedvaluableinsightsintothecontributionsoftheCpxsystemtothestressresponseandvirulenceofDBHS101.ThisresearchimprovesourunderstandingofSphingomonasaquaticastrainDBHS101andhighlightstheimportanceoftheCpxsysteminbacterialphysiology. Introduction: SphingomonasaquaticastrainDBHS101isagram-negativebacteriumbelongingtotheSphingomonadaceaefamily.Itisanaerobicbacteriumknownforitsabilitytoutilizeawiderangeoforganiccompoundsanditspotentialapplicationinenvironmentalbioremediation.TheCpxsystemisatwo-componentsignaltransductionpathwayresponsibleforsensingandrespondingtoenvelopestressinbacteria.Itplaysanessentialroleinregulatingproteinhomeostasisandbacterialvirulence.Inthisstudy,weaimedtoanalyzethegeneticcompositionofDBHS101andinvestigatetheroleoftheCpxsysteminitsbiologicalcharacteristics. Methods: 1.GenomicAnalysis:ThegenomeofDBHS101wassequencedusinghigh-throughputsequencingtechnology.Bioinformaticanalysiswasperformedtoidentifygenesencodingkeyenzymesandtransportersinvolvedinwateradaptationandenvironmentalremediation. 2.ConstructionofCpx-deficientMutants:AmarkerlessgenedeletionsystemwasemployedtoconstructCpx-deficientmutants.ThecpxRandcpxAgenesweretargetedfordeletiontodisrupttheCpxsystem'sfunction. 3.PhenotypicAnalysis:ThephenotypiccharacteristicsoftheCpx-deficientmutantswereinvestigated,includinggrowthrate,biofilmformation,motility,andstresstolerance.Com