微流控分析芯片.ppt

第八章微流控分析芯片Doyouknow?Researchintominiaturizationisprimarilydrivenbytheneedtoreducecostsbyreducingtheconsumptionofexpensivereagentsandbyincreasingthroughputandautomation.Forexample,mostareawareoftheincreasingcostofhealthcare,driveninpartbythecostofimplementingthelatestdiagnosticassays.Theseassays,whichareusuallyperformedinmicrotiterplatesthatconsumehundredsofmicrolitersofreagents,wouldbenefitfromtheuseofmicrofabricatedarraysofnanolitervolumevials.Byreducingreagentconsumptionbyafactorof103–104,thesedevicescouldprovidedramaticsavingsfortherepetitiveassaysoftenperformedindiagnosticlaboratories.尺寸效应（cm→100m）
尺寸 1/100
分子扩散时间 1/10,000(1h→0.36s)
体积 1/1,000,000
试剂用量 1t→1g,ml→nl
传热速度 1500oC/s
由此将带来：
方法上的变革
理论上的突破
还有巨大的经济和社会效益阵列芯片微流控分析芯片参考文献目录TheEarlyDays:1975-1989However,theresponseofthescientificcommunitytothisfirstsiliconchipdevicewasvirtuallynone,presumablybecauseofthelackoftechnologicalexperience(oftheseparationscientists)todealwiththiskindofdevice.
theresearchworkrelatedtominiaturizationonsiliconfocusedonthefabricationofcomponentssuchasmicropumps,microvalves,andchemicalsensors.TheRenaissance:1990-1993GrowingtoCriticalMass:1994-1997分类与特点8-2相关理论8-2-1基本概念液体流动的特点液体流动的特点Aq.扩散的数字模拟不同尺寸通道的数字模拟微结构的形成1．经典的光刻技术微结构的形成2．模版浇注法（模塑法）微结构的形成3．模版热压法微结构的形成4．激光刻蚀法芯片的封装1．热键合芯片的封装2．阳极键合芯片的封装3．室温键合芯片的封装4．贴合将聚合物薄片直接覆盖在玻璃或石英板上。5．压合表面修饰1．作为色谱固定相表面修饰2．固定化酶表面修饰3．固定化生物膜表面修饰4．亲水或疏水修饰与外部溶液连接与质谱仪连接外加压力驱动重力驱动离心力驱动毛细作用驱动利用电渗流驱动与控制利用表面性质控制单向阀8-4单元操作8-4-1样品制备SonicationAmicrofluidiccartridgetopreparesporesforPCRanalysisExtractionConceptofsequentialion-sensingsystemusingsinglemicrochipSchematicillustrationofexperimentalsetupandionpairextractionmodeldeterminationofCo(II)as2-nitroso-1-naphtholchelatesbysolventextractionandthermallensmicroscopyPreconcentrationElectrokineticPreconcentrationofProteinsonthinPDMSMembranesSchematicdiagramsofCEprocedureswithpinchedinjection(toppanels),floatinginjection(middle),and(C)simplestinjectionmode(bottom).Schematicofmicrochipusedforgatedvalving.Thedoublelinesrepresentm