预览加载中，请您耐心等待几秒...

贫营养湖泊花神湖和紫霞湖浮游细菌群落结构分析.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

贫营养湖泊花神湖和紫霞湖浮游细菌群落结构分析 Introduction Nutrientpoorlakes,alsoknownasoligotrophiclakes,haveextremelylowlevelsofnutrients,includingnitrogenandphosphorus,whichlimitsthegrowthofalgaeandotheraquaticplantsandorganisms.Despitetheirlimitednutrientsupply,theselakescansustaindiverseandproductiveecosystems,astheyoftenprovidehabitatforavarietyoffishandwildlifespecies.TwoexamplesofoligotrophiclakesareHuashenhuLakeandZixiaLake,locatedinChina. Inthispaper,wewillanalyzethestructureofthephytoplanktoncommunityinHuashenhuandZixialakes,focusingontheroleoffloatingbacteriaandtheirinteractionswithotherorganismsintheseecosystems. Methods ToanalyzethestructureofthephytoplanktoncommunityinHuashenhuandZixialakes,watersampleswerecollectedfromvariousdepthsusingaVanDornwatersampler.Sampleswerethenfilteredthrougha0.22μmporesizefiltertoremoveallcellslargerthanbacteria. DNAextractionandsequencingwereperformedusingIlluminaMiSeqsystemandprimerstargetingtheV4hypervariableregionofthe16SrRNAgene.Theresultingsequenceswereanalyzedusingbioinformatictoolstodeterminethebacterialcommunitystructureanddiversity. Results TheanalysisofthebacterialcommunitystructureinHuashenhuandZixialakesrevealedahighdiversityofbacterialtaxa,withthemostabundantphylabeingProteobacteria,Bacteroidetes,andActinobacteria.Thedistributionofbacterialtaxavariedbydepthandlocationinthelakes,withsometaxabeingmoreprevalentinspecificareas. Inadditiontothediversityofbacterialtaxa,thephytoplanktoncommunityintheselakeswasfoundtobedominatedbydiatoms,greenalgae,andcyanobacteria.Thesegroupsoforganismswerefoundtointeractwiththefloatingbacteriaincomplexways,withsomebacteriaprovidingnutrientsandotherresourcestothephytoplankton,whileothersmaycompetefortheseresources. Conclusion TheanalysisofthephytoplanktoncommunitystructureinHuashenhuandZixialakesrevealedacomplexandhighlydiverseecosystem,supportedbyacombinationoffloatingbacteriaandphytoplanktonspecies.Thisstudyprovidesvaluableinsightsintotheroleofbacteriainoligotrophiclakes,andhighlightstheimportanceofcon