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葡萄糖氧化酶在丝素膜中的固定化及其性能和应用于生物传感器(英文).docx

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葡萄糖氧化酶在丝素膜中的固定化及其性能和应用于生物传感器(英文) Glucoseoxidase(GOx)isanimportantenzymewidelyusedinvariousfields,includingbiosensors,foodindustry,andmedicalapplications.Inthispaper,wefocusontheimmobilizationofGOxonsilkfibroinmembraneanditspropertiesandapplicationsinbiosensors. ImmobilizationofGOxonsilkfibroinmembrane TheimmobilizationofGOxonsilkfibroinmembranecanbeachievedbyseveralmethods,includingphysicaladsorption,covalentbinding,andentrapment.Amongthesemethods,covalentbindingisthemostcommonlyusedmethodduetoitshighstabilityandefficientcatalyticactivity. ToimmobilizeGOxonsilkfibroinmembrane,firstly,thesilkfibroinmembranewasactivatedbytreatingitwithglutaraldehyde.Then,theactivatedsilkfibroinmembranewasincubatedwithGOx,andthecouplingreactionwascarriedoutundermildconditions.Finally,theimmobilizedGOxonsilkfibroinmembranewaswashedseveraltimestoremovetheunboundenzymemoleculesanddriedatroomtemperature. PropertiesofimmobilizedGOx TheimmobilizedGOxonsilkfibroinmembraneexhibitsseveralpropertiesthataredifferentfromthefreeGOxinsolution.Firstly,theimmobilizedGOxhasahigherthermalstability,whichisduetothehighstabilityofsilkfibroinmembraneandthestrongcovalentbondbetweenGOxandsilkfibroin.Secondly,theimmobilizedGOxhasahigherstoragestability,whichisbeneficialforlong-termstorageandpracticalapplications.Thirdly,theimmobilizedGOxexhibitsahigherresistancetopHanddenaturants,whichisimportantformaintainingitscatalyticactivityinharshconditions. ApplicationsofimmobilizedGOxinbiosensors TheimmobilizedGOxonsilkfibroinmembranehasbeenwidelyusedinbiosensorsforglucosedetection.Itcancatalyzetheoxidationofglucoseinthepresenceofoxygen,andthegeneratedhydrogenperoxidecanbedetectedelectrochemically.TheimmobilizedGOx-basedbiosensorshaveseveraladvantages,includinghighsensitivity,selectivity,andstability,andhavefoundapplicationsinclinicaldiagnosis,foodindustry,andenvironmentalmonitoring. Forexample,aGOx-basedbiosensorwasdevelopedforthedetectionofglucoseinhumanserumusingasilkfibroinmembraneasasupportingmaterial.Thebiosensor