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碎米双酶法制备葡萄糖的工艺研究(英文) Title:ProcessStudyofGlucoseProductionbyAmylaseHydrolysisMethod Abstract: Glucoseisanimportantmonosaccharidethatservesasavitalsourceofenergyforlivingorganisms.Themanufacturingofglucoseinvolvesvariousmethods,andonesuchapproachistheamylasehydrolysismethod.Thispaperaimstoprovideanin-depthexplorationoftheprocessinvolvedinproducingglucosethroughthefragmentationmethodbyamylase. 1.Introduction Glucose,asaprimaryenergysource,playsacrucialroleinbothbiologicalsystemsandawiderangeofindustrialapplications.Traditionalmethodsofglucoseproductioninvolveacidhydrolysisofstarch,whichhasseveraldisadvantagessuchasenvironmentalpollutionandhighcosts.Theamylasehydrolysismethod,ontheotherhand,hasgainedsignificantattentionduetoitshighefficiencyandenvironmentalfriendliness.Thisstudyfocusesontheuseofamylasetobreakdownstarchintoglucosethroughthefragmentationprocess. 2.OverviewofAmylaseHydrolysisMethod Amylaseisanenzymethatcatalyzesthehydrolysisofstarch,breakingitdownintomaltoseandglucose.Theamylasehydrolysismethodinvolvestheuseofamylaseenzymestoinitiatethefragmentationofstarchmoleculesintosmallerchains.Theseshorterchainsarethenfurtherhydrolyzedintoglucosebyotherenzymesortreatmentwithacids. 3.FactorsAffectingAmylaseHydrolysis Severalfactorsinfluencetheefficiencyoftheamylasehydrolysisprocess.Theseincludetemperature,pH,substrateconcentration,enzymeconcentration,andreactiontime.Optimalconditionsforeachparameterneedtobedeterminedtomaximizetheyieldofglucose. 4.ProcessOptimization Variousstrategiescanbeemployedtooptimizetheamylasehydrolysisprocess.Thisincludesselectingtheappropriatetypeofamylaseenzyme,optimizingreactionconditions,andinvestigatingtheeffectofadditivesonthehydrolysisefficiency.Theuseofimmobilizedenzymesorenzymereactorscanalsoenhancetheprocessefficiency. 5.ComparisonwithOtherGlucoseProductionMethods Incomparisontotraditionalacidhydrolysismethods,theamylasehydrolysismethodoffersseveraladvantages.Ithasmorepreferableenvironmentalcharacteristics,asitdoesnotproducetoxicbypro