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代谢工程改造大肠杆菌提高乙醇酸产率.docx

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代谢工程改造大肠杆菌提高乙醇酸产率 Title:MetabolicEngineeringofEscherichiacoliforEnhancedLacticAcidProduction Abstract: Lacticacidisawidelyusedorganicacidwithapplicationsinvariousindustriessuchasfood,pharmaceutical,andchemical.Efficientproductionoflacticacidisofgreatsignificanceformeetingincreasingmarketdemands.ThispaperfocusesonmetabolicengineeringapproachestoenhancelacticacidproductioninEscherichiacolibyoptimizingkeymetabolicpathwaysandimprovingfermentationperformance.ThestudyhighlightsthestrategiesemployedtoincreaseNADHavailability,redirectcarbonflux,andimprovesubstrateuptake,alongwiththeevaluationoftheeffectsofdifferentfermentationconditions.ThefindingspresentedinthispapercontributetotheunderstandingofthemetabolicpotentialofE.coliforlacticacidproductionandofferinsightsintothedevelopmentofmoreefficientmicrobialcellfactoriesforindustrialapplications. Introduction: Lacticacid(2-hydroxypropanoicacid)isakeymetaboliteproducedthroughmicrobialfermentation.Thedemandforlacticacidhasbeensteadilyincreasingduetoitsdiverseapplicationsinthefood,pharmaceutical,andchemicalindustries.Escherichiacoli,awidelystudiedmodelmicroorganism,hasshowngreatpotentialasaplatformforlacticacidproductionduetoitsrobustness,well-characterizedgenetics,andextensivemetabolicengineeringtoolkit. MetabolicEngineeringStrategiesforEnhancedLacticAcidProduction: 1.IncreasingNADHavailability: LacticacidproductionisdependentontheavailabilityofNADH,whichservesasacofactorforthelactatedehydrogenase(LDH)enzyme.Therefore,engineeringstrategiesaimingtoincreaseNADHavailabilityhavebeenemployed.OneapproachistheoverexpressionofNADH-producingenzymes,suchaspyruvatedehydrogenasecomplex(PDH),whichgeneratesNADHfrompyruvate.AnotherstrategyinvolvestheinhibitionofNADH-consumingpathways,suchastheacetateproductionpathway,toredirectNADHtowardslacticacidproduction. 2.Redirectingcarbonflux: Optimizingthedistributionofcarbonfluxtowardslacticacidsynthesisiscrucialforenhancingproduction.Thiscanbeachievedbymanipulatingkeyenzymesinvolvedincentralcarbonmet