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丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析.docx

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丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析 Title:EffectsofArbuscularMycorrhizalFungiandRhizobiaCo-inoculationontheBacterialCommunityStructureandPredictedFunctioninAlfalfaRhizosphereSoil:APICRUStAnalysis Abstract: Themicrobialinteractionsinplantrootsystemsplayacrucialroleinplantgrowthanddevelopment.Thisstudyaimedtoinvestigatetheeffectsofarbuscularmycorrhizal(AM)fungiandrhizobiaco-inoculationonthebacterialcommunitystructureandpredictedfunctionsinalfalfarhizospheresoilusingthePhylogeneticInvestigationofCommunitiesbyReconstructionofUnobservedStates(PICRUSt)analysis.Theresultsrevealedsignificantchangesinthebacterialcommunitycompositionandpredictedfunctionalprofilesinresponsetothesymbioticinteractions.Thisresearchcontributestoacomprehensiveunderstandingofthecomplexmicrobialinteractionsinagriculturalecosystemsandprovidesinsightsintothepotentialapplicationsofmicrobialinoculantsforsustainablecropproduction. Introduction: Symbioticinteractionsbetweenplantsandmicroorganisms,suchasarbuscularmycorrhizal(AM)fungiandrhizobia,significantlyinfluenceplanthealthandecosystemfunctioning.AMfungiformmutualisticassociationswiththerootsofmostterrestrialplants,whilerhizobiaestablishsymbioticrelationshipswithleguminousplants,includingalfalfa(MedicagosativaL.),byformingrootnodules.Thesemicrobialinteractionsnotonlyenhancenutrientacquisitionandstresstolerancebutalsoaffectthecompositionandfunctioningoftheroot-associatedbacterialcommunity.Understandingtheimpactsoftheseinteractionsonthemicrobialcommunitystructureandpredictedfunctionsiscrucialforelucidatingthemechanismsunderlyingplant-microbeinteractionsandimprovingagriculturalsustainability. Methods: Inthisstudy,weconductedapotexperimentusingalfalfaplantsinoculatedwithAMfungi,rhizobia,oracombinationofboth.Rhizospheresoilsampleswerecollected,andbacterialcommunityanalysiswasperformedusinghigh-throughputsequencingofthe16SrRNAgene.ThePICRUStapproachwasemployedtopredictthefunctionalpotentialsofthebacterialcommunitiesbasedontheobtained16SrRNAgenedata. Re