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Streptomycesalboflavus抗真菌物质对串珠镰刀菌的抑制机理研究.docx

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Streptomycesalboflavus抗真菌物质对串珠镰刀菌的抑制机理研究 Abstract: Streptomycesalboflavusisawell-knownproducerofbioactivesecondarymetabolites,includingantimicrobialcompounds.Inthisstudy,weinvestigatetheinhibitorymechanismoftheStreptomycesalboflavusantimicrobialsubstanceagainstFusariumoxysporum,afilamentousfungusthatcausesseriouscropdiseases.Throughaseriesofexperimentsandanalyses,weidentifiedthespecificmodesofactionfortheantimicrobialcompound,sheddinglightonitspotentialuseasanaturalandsustainablefungicide. Introduction: Fusariumoxysporumisaplantpathogenicfungusthatcauseswiltdiseasesinnumerouscrops,resultinginsignificanteconomiclossesworldwide.Traditionalfungicidesareoftenusedtocombatthisdevastatingpathogen;however,theireffectivenessislimitedandtheyposepotentialriskstotheenvironmentandhumanhealth.Therefore,thesearchforalternativeandmoresustainablecontrolstrategies,suchasthedevelopmentofbiocontrolagents,isofgreatimportance. Streptomycesalboflavus,asoil-dwellingactinobacterium,isknownforitsproductionofsecondarymetaboliteswithantimicrobialproperties.Previousstudieshavedemonstratedthebroad-spectrumantimicrobialactivityofS.alboflavus-derivedcompounds,includingpotentinhibitionofvariousfungalspecies.Inthisstudy,wefocusontheinhibitorymechanismofS.alboflavusagainstF.oxysporum. MaterialsandMethods: 1.IsolationandcharacterizationofStreptomycesalboflavusstrains:Severalsoilsampleswerecollectedfromdifferentlocations,andStreptomycesstrainswereisolatedusingtraditionalmicrobiologicaltechniques.Thestrainswerethenidentifiedbasedonmorphologicalandmolecularcharacterizationmethods. 2.Antimicrobialcompoundextractionandpurification:TheStreptomycesalboflavusstrainswereculturedinsuitablegrowthmediatoinducetheproductionofsecondarymetabolites.Theantimicrobialcompoundswereextractedusingorganicsolvents,followedbypurificationusingchromatographictechniques. 3.Inhibitionassay:TheantifungalactivityoftheS.alboflavuscompoundswasdeterminedusingadiscdiffusionassay.F.oxysporumcoloniesweregrownonagarplates,andsterilefilter