以水解液为原料的α-淀粉酶发酵工艺条件的研究.docx

以水解液为原料的α-淀粉酶发酵工艺条件的研究
摘要
本文以水解液为原料，通过单因素实验和响应面实验优化了α-淀粉酶的发酵条件。结果表明，最佳条件为发酵温度35℃、发酵时间48h、初始pH值为6.5、α淀粉酶添加量为2.5g/L。在此条件下，α-淀粉酶的产酶量为690.45U/mL。最后，应用此条件进行了扩大实验，获得了较高的产量和活力。
关键词：α-淀粉酶；发酵条件；水解液；响应面实验
Introduction
Alpha-amylasesareagroupofenzymeswhichcatalyzethehydrolysisofα-1,4-glycosidicbondsofstarch,glycogen,andrelatedpolysaccharides.Theyarewidelyusedinmanyindustries,suchasfood,fermentation,andtextile.Asaresult,itisimportanttooptimizeα-amylaseproductionprocesses.Inthisstudy,weaimedtooptimizethefermentationconditionsofα-amylaseusinghydrolysateastherawmaterial.
Materialsandmethods
α-amylaseproducingstrainBacillussubtiliswasusedinthisstudy.Hydrolysatewaspreparedbyhydrolyzingcornstarchwithhydrochloricacid.Singlefactorexperimentswerecarriedouttodeterminetheeffectofvariousfactorsonα-amylaseproduction.Thefactorsstudiedincludedtemperature,pH,fermentationtime,andα-amylaseconcentration.Acentralcompositedesignwasthenemployedtofurtheroptimizethefermentationprocess,andaresponsesurfacemethodologywasusedtoanalyzetheresults.
Results
Theresultsofsinglefactorexperimentsshowedthattheoptimaltemperatureforfermentationwas35℃,theoptimalpHwas6.5,theoptimalfermentationtimewas48hours,andtheoptimalα-amylaseconcentrationwas2.5g/L.Thecentralcompositedesignshowedthattheoptimalconditionswereatemperatureof35℃,pHof6.5,fermentationtimeof48hours,andα-amylaseconcentrationof2.5g/L.Undertheseconditions,theα-amylaseproductionwas690.45U/mL.Theseresultswereconfirmedintheenlargedexperiment,whichyieldedahighproductionandactivity.
Discussion
Ourresultssuggestthatagoodα-amylasefermentationprocesscanbeachievedwithhydrolysateastherawmaterial.Theoptimalfermentationconditionsweredeterminedtobeatemperatureof35℃,pHof6.5,fermentationtimeof48hours,andα-amylaseconcentrationof2.5g/L.Theseresultsareconsistentwithpreviousstudiesonα-amylasefermentationconditions.Theresultsofthestudysuggestthatoptimizationoffermentationconditionscangreatlyincreasetheproductionandactivityofα-amylase.
Conclusion
Optimizationofα-amylasefermentati