黑曲霉菌株产纤维素酶的固态发酵条件优化.docx

黑曲霉菌株产纤维素酶的固态发酵条件优化
摘要
本文旨在优化黑曲霉菌株产纤维素酶的固态发酵条件，通过单因素实验和正交试验评估了发酵温度、pH值、初始水分含量和发酵时间对酶活力的影响。实验结果显示，最优发酵条件为发酵温度为45℃、pH值为6.5、初始水分含量为60%、发酵时间为72小时，此条件下纤维素酶活力可达到15.62IU/g。优化后的固态发酵条件有望用于实际纤维素酶的产量提高和工业应用。
关键词：黑曲霉菌；纤维素酶；固态发酵；条件优化
Abstract
Theobjectiveofthisstudywastooptimizethesolid-statefermentationconditionsofablackAspergillusstrainforcellulaseproduction.Theeffectsoffermentationtemperature,pHvalue,initialmoisturecontent,andfermentationtimeonenzymeactivitywereevaluatedbysingle-factorexperimentsandorthogonalexperiments.Theresultsshowedthattheoptimalfermentationconditionswereafermentationtemperatureof45℃,apHvalueof6.5,aninitialmoisturecontentof60%,andafermentationtimeof72hours,andthecellulaseactivitycouldreach15.62IU/g.Theoptimizedsolid-statefermentationconditionswereexpectedtobeappliedincellulaseproductionandindustrialapplications.
Keywords:blackAspergillus;cellulase;solid-statefermentation;conditionoptimization
1.Introduction
Cellulaseisakindofhydrolyticenzymethatcandegradecelluloseintoglucose,whichiswidelyusedinvariousindustriessuchasfood,papermaking,bioenergy,andtextile[1].Withthegradualdepletionoffossilresourcesandtheincreasingawarenessofenvironmentalprotection,thedemandforbiologicallyproducedcellulaseisontherise.However,thehighcostofenzymeproductionhasbecomethebottleneckofindustrialization.Therefore,itisnecessarytofindhigh-yieldandlow-costenzymeproductiontechnology.Solid-statefermentationisapromisingmethodforcellulaseproductionduetoitsadvantagesoflowcost,highenzymeactivity,andhightolerancetoinhibitors[2].
BlackAspergillusisacommonfilamentousfunguscapableofproducingavarietyofcellulose-degradingenzymes[3].Ithasreceivedincreasingattentioninrecentyearsduetoitsefficientcellulaseproduction.However,theproductionofcellulasebyblackAspergillusisaffectedbymanyfactors,suchasfermentationtemperature,pHvalue,initialmoisturecontent,andfermentationtime,whichneedtobeoptimizedtoimprovetheenzymeyield.
Therefore,thisstudyaimedtooptimizethesolid-statefermentationconditionsofablackAspergillus