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响应面法优化月季花中总黄酮的提取工艺.docx

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响应面法优化月季花中总黄酮的提取工艺 Title:OptimizationofTotalFlavonoidExtractionProcessfromRosarugosaUsingResponseSurfaceMethodology Abstract: Rosarugosa,commonlyknownastherugosaroseortheJapaneserose,isapopularfloweringplantthatisrichinbioactivecompoundsincludingflavonoids.Flavonoidsareagroupofsecondarymetabolitesknownfortheirantioxidantandanti-inflammatoryproperties.Inthisstudy,thetotalflavonoidextractionprocessfromRosarugosawasoptimizedusingresponsesurfacemethodology(RSM).Variousfactorsaffectingtheextractionefficiencywereanalyzed,includingextractiontime,extractiontemperature,solvent-to-solidratio,andethanolconcentration.TheresultsshowedthattheRSMcouldeffectivelyoptimizetheextractionprocesstoobtainmaximumtotalflavonoidyieldfromRosarugosa. 1.Introduction: 1.1Background: Flavonoidsareadiversegroupofplantsecondarymetabolitesthatarewidelydistributedinfruits,vegetables,andmedicinalplants.Theyhaveattractedsignificantattentionduetotheirpotentialhealthbenefits,includingantioxidant,anti-inflammatory,anti-cancer,andcardioprotectiveactivities. 1.2Rosarugosa: RosarugosaisaspeciesofrosenativetoEastAsia.Itisaperennialshrubwithattractiveflowersandediblefruits.Thisplanthasbeenwidelyusedintraditionalmedicineforitsvariouspharmacologicalactivities.Severalstudieshavereportedthepresenceofnumerousbioactivecompounds,especiallyflavonoids,inRosarugosa. 1.3TotalFlavonoidExtraction: Theextractionofflavonoidsfromplantmaterialsisacrucialsteptoobtainthesebioactivecompoundsinconcentratedform.Severalextractionmethodshavebeenemployed,includingconventionalmaceration,ultrasound-assistedextraction,microwave-assistedextraction,andsoon.However,optimizingtheextractionprocessisessentialtomaximizetheyieldoftargetedcompounds. 2.Methodology: 2.1ExperimentalDesign: Responsesurfacemethodology(RSM)isastatisticaltechniqueusedtooptimizeprocessparametersbygeneratingmathematicalmodels.Inthisstudy,acentralcompositedesign(CCD)wasemployedtooptimizethetotalflavonoidextractionprocessfromRosarugosa.Thefactorsinvestigatedwereextractionti