预览加载中，请您耐心等待几秒...

响应面法优化酿酒酵母高产谷胱甘肽的发酵条件.docx

预览
1/3
2/3
3/3

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

响应面法优化酿酒酵母高产谷胱甘肽的发酵条件 Title:OptimizationofFermentationConditionsfortheHigh-YieldProductionofGlutathioneinBrewingYeastusingResponseSurfaceMethodology Abstract: Theproductionofglutathione,apotentantioxidantandmultifunctionalmoleculewithvariousindustrialapplications,hasgarneredsignificantinterestinrecentyears.Thisstudyaimstooptimizethefermentationconditionsforthehigh-yieldproductionofglutathioneinbrewingyeastusingtheresponsesurfacemethodology(RSM).RSMallowsfortheefficientexplorationanddeterminationofoptimalprocessparametersbyconsideringtheinteractioneffectsofmultiplevariables.Inthisresearch,weinvestigatedandoptimizedtheeffectsofpH,temperature,agitationspeed,andglucoseconcentrationonglutathioneproduction.TheresultsdemonstratetheeffectivenessofRSMinoptimizingfermentationconditions,leadingtoanincreasedproductionofglutathioneinbrewingyeast. Keywords:Glutathione,Brewingyeast,Fermentationconditions,Responsesurfacemethodology,Optimization 1.Introduction: Glutathione(GSH)isatripeptidecomposedofglutamate,cysteine,andglycine.Itsroleasanantioxidantandcellularprotectorhasledtoitswideapplicationsinthepharmaceutical,food,andcosmeticindustries.Brewingyeast,withitsrobustmetabolismandabilitytoproducehigh-valuecompounds,providesanexcellentplatformfortheproductionofglutathione.However,theoptimizationoffermentationconditionsisnecessarytoachievehigh-yieldproduction.Thisstudyaimstoutilizetheresponsesurfacemethodology(RSM)tooptimizethefermentationconditionsandenhanceglutathioneproductioninbrewingyeast. 2.MaterialsandMethods: 2.1.MicroorganismandCultureConditions: Asuitablestrainofbrewingyeastwasselectedfortheproductionofglutathione.Theyeastwasculturedinasyntheticmediumcontainingglucose,yeastextract,andpeptone. 2.2.ExperimentalDesign: Acentralcompositedesign(CCD)consistingof29experimentalrunswasemployedtoinvestigatetheeffectsoffourindependentvariables(pH,temperature,agitationspeed,andglucoseconcentration)onglutathioneproduction.Therangeofeachvariablewasdeterminedbasedonpriorexperimentalknowle